Objective Employee satisfaction is closely related to the medical service quality of the hospital. By investigating the current employee satisfaction of a children′s hospital in Jinan, the key restricting factors affecting the improvement of human resources in children′s specialized hospital were found out, so as to facilitate the establishment of reasonable measures and systems. Methods According to the characteristics of specialized children′s hospitals and the performance assessment requirements of tertiary public hospitals of the National Health Commission, a questionnaire on staff satisfaction of tertiary public children′s hospitals with 23 indicators was developed. An anonymous questionnaire was used to investigate all the current employees of the hospital, and SPSS 19.0 software was used for analysis. Results The overall satisfaction score of the present employees was 3.81±0.49. The most volatile categories focused on the work environment, the relationship between superiors and coworkers, and job satisfaction. Employees with primary and intermediate titles scored 3.83 (3.43, 4.22) M(P₂₅～P₂₅) mainly due to the large gap between their salary and work pressure. The scores of doctors and pharmacists were low, respectively 3.70 (3.43～4.04) and 3.63 (3.36, 4.00), which were consistent with the current situation of talents in children′s specialized hospitals in China. Conclusion The comprehensive quality of staff, work pressure and management level have become the factors restricting the development of the hospital, so it is necessary to strengthen the publicity, improve the doctorpatient relationship and perfect the working process, so as to promote the cultivation and construction of the talent team related to pediatrics.