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| Construction, phenotype and preliminary application of SUMO1 T95R mutation mouse model |
| LIU Xiuxiu, ZHANG Chao, ZHOU Yizi, TAN Ying, MI Jia, JIANG Wenguo* |
| Department of Pharmacy, Binzhou Medical University, Yantai 264003, Shandong, P.R.China |
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Abstract Objective To identify the SUMO-modified sites of myosin by constructing a T95R mutation SUMO1 mouse model in mice.Methods Homologous recombination was used to estabish mutations in ES cells and construct the T95R point mutation SUMO1 mouse model. Mouse gene identification technology was used to identify the T95R mouse genetic mutation.The weight and blood glucose were detected of T95R mutation SUMO1 mouse. The myosin purification and proteomics were used to identify SUMO-modified sites.Results The T95R mouse model was established successfully by the mouse gene identification technology. The T95R site mutation homozygous mice and wild-type mice had the same trends in body weight and blood glucose through quantitative analysis. Proteomics technology showed that T95R homozygous mice could be detected different novel SUMO-modified myosin sites.Conclusion This study constructed a special SUMO1 mutation mouse model to provide a simple and feasible model for the identification of SUMOylation.The discovery of new SUMOylation sites is helpful to study the physiopathological function of myosin.
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Received: 08 June 2020
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