15 February 2020, Volume 43 Issue 1

    

• Select all
  |

• Select
  Effect of umbilical cord mesenchymal stem cells on the regulation of apoptosis of theca cells by NR4A1 in primary ovarian insufficiency

  BAI Lanlan, LUO Qianqian, LIU Ranran, ZHANG Hongqin

  滨州医学院学报. 2020, 43(1): 1-7. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.001

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To investigate the therapeutic effects of umbilical cord mesenchymal stem cells (UCMSCs) on (primary ovarian insufficiency) POI rats and the mechanism of NR4A1 expression in the theca-interstitial cells (TICs) during the treatment. Methods Female Wistar rats of 5 weeks old were randomly divided into control group, POI group and POI+UCMSCs group. POI group was intraperitoneally injected with cis-platin (CP), and the control group was intraperitoneally injected with 0.9% saline, following with 7 days. POI+UCMSCs were treated with CP for 1 week, and then UCMSCs suspension was injected into the tail vein. One week later, the levels of E₂ and FSH in the serum of the three groups were detected by enzyme-linked immunosorbent assay (ELISA). The morphological changes of ovaries were observed by HE staining. The apoptosis of granulosa
  cells was detected by TUNEL. In addition, TICs were cultured in vitro and NR4A1 was expressed in TICs. CP and UCMSCs were treated into TICs and western blot was used to detect the levels of NR4A1 expression. Annexin V-FITC apoptosis assay kit was used to detect the apoptosis of TICs. Results Compared with the control group, the ovary of POI group was atrophy and the number of follicles in the ovary was significantly reduced(P<0.05), following the “luteal-like” structure being increased significantly(P<0.05). The number of follicles in the ovary increased significantly after UCMSCs treatment(P<0.05). Compared with the control group,the serum E₂ level in the POI group was significantly decreased(P<0.01), while the FSH level was significantly increased(P<0.05). Compared withthe POI group, the serum E₂ level was increased and the FSH level was decreased by UCMSCs treatment(P<0.05). Compared with the control group, the apoptosis of the granulosa cells in the POI group was significantly increased, while the UCMSCs could decrease the apoptosis of the granulosa cells(P<0.01). In addition, TICs cultured in vitro were fusiform and Cyp17a1 and NR4A1 were expressed, but FSHR expressed negative(P<0.05). After CP treatment, the NR4A1level was significantly decreased, and the apoptosis of TICs was significantly increased. After treatment with UCMSCs, the expression of NR4A1 was increased, but the apoptosis of TICs was significantly decreased(P<0.01). Conclusion UCMSCs could restore the ovarian function of POI by regulating the NR4A1 level of TICs expression and increase the regulation of TICs on granulosa cell apoptosis. This could provide a certain theoretical and experimental basis about the treatment of UCMSCs in POI.
• Select
  The study of the treatment of tinnitus with hydroxysafflor yellow A

  MA Chunlei, SONG Shaoqing

  滨州医学院学报. 2020, 43(1): 8-10. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.002

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To study the effect of hydroxysafflor Yellow A(HSYA)on the salicylated-induced tinnitus. Methods Twenty-four mice were divided into three groups, which were given respectively normal saline (0.4 mL), sodium salicylate (400 mg/kg) and sodium salicylate combined with HSYA (20 mg/kg) by peritoneal injection (i.p.) one time a day for 3 days continuous. After 2 h of administration, the ratio of acoustic startle (AS) response amplitude was detected, and the background noise were 8 and 16 kHz narrow-band noise. Results The mice in sodium salicylate injection group showed obvious tinnitus-like behavior. At 16 kHz, compared with the sodium salicylate group, the amplitude of AS response of mice in the sodium salicylate combined with HSYA group was significantly decreased(P<0.05). Conclusion HSYA can effectively prevent tinnitus caused by sodium salicylate.
• Select
  Application of quantitative dot blot analysis technology for protein expression level detection

  LIU Lin, MEN Tingting, ZHANG Wenfeng, ZHANG Jiandi, MI Jia, YANG Chunhua

  滨州医学院学报. 2020, 43(1): 11-14. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.003

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To detect the application of quantitative dot blot analysis (QDB analysis) detection technology for protein expression level analysis by determining the protein level of high throughput tissue samples and cell samples. Methods The relative expression of tubulin in the prostate tissues from 87 mice and the absolute expression of tubulin from 8 human cell lines were detected by QDB analysis. Results The results showed that the expression level of tubulin protein in prostate tissue from mice was different according to the age, and the absolute expression level of tubulin protein in 8 cell lines was between 90～140 fmol/μg. Conclusion QDB analysis can be applied to the relative and absolute quantification in a high throughput tissue samples and cell samples.
• Select
  Clinical research on the ultimate treatment of adult tibial shaft closed fracture with external fixator

  WANG Chuanjin, LI Peng, YANG Shuye, ZHANG Kai

  滨州医学院学报. 2020, 43(1): 15-18. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.004

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To investigate the clinical effect of external fixator in the treatment of adult tibial shaft closed fracture. Methods Forty-seven adult patients with tibial shaft closed fracture were treated with external fixator. An external fixation framework was designed according to the type of fracture before surgery, and an external fixation frame was installed after the intraoperative X-ray fluoroscopy machine confirmed the closed reduction fracture was satisfactory. Nursing of nail track was strengthened after operation to prevent infection and all patients were followed up at regular intervals. According to the condition of bone healing mineralization shown in X-ray films, components of the frame were gradually dismantled to total remove of the frame in regular intervals. Results Forty-seven patients were followed up 12 to 36 months, with an average of (19.96±1.42) months. Forty-five cases of 47 patients healed normally and 2 patients had nonunion, with the healing rate 95.74%. The fracture healing time was from 4 to 14 months, with an average of (5.63±0.78) months. One case of nonunion healed smoothly after replacing plate and bone grafting, and the other one healed smoothly after Bone transport technique with ring external fixator. Three patients with mild pin-tract infection were cured after debridement and changing dressing associated with antibiotics. There were no impacts on the knee and ankle function in all patients. Conclusion External fixator is the ultimate treatment for closed fracture of tibial shaft in adults with satisfactory clinical efficacy. The appropriate external fixator can provide stable and reliable fixation strength for fracture healing, and can adjust the fixation strength according to the bone healing situation to improve stress shielding and avoid secondary surgical treatment, which has certain clinical application value.
• Select
  Preoperative precise localization of cervical disc herniation by brachial plexus MRN in the treatment of cervical spondylotic radiculopathy

  HUANG Zhen, LI Yuefei, BI Jingwei, REN Jiabin, LI Rui, LIU Xin, SUN Ning, SUN Zhaozhong

  滨州医学院学报. 2020, 43(1): 19-22. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.005

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To analyze the clinical efficacy of precise decompression in posterior percutaneous endoscopic cervical discectomy (PPECD) by locating the herniated cervical disc through brachial plexus magnetic resonance neurography (MRN). Methods Thirty-nine patients with cervical spondylotic radiculopathy (CSR) who underwent PPECD were divided into MRN group and conventional group according to whether brachial plexus MRN was performed before operation. There were 18 patients in MRN group and 21 patients in control group. The operation time, bleeding volume, fluoroscopy times during operation, incidence of upper limb numbness and muscle weakness after operation, the longest diameter of left and right bone window and the longest diameter of upper and lower bone window were recorded. The VAS and JOA value of preoperative, postoperative, 6 months after operation and 12 months after operation were compared between the two groups. C2～C7 Cobb angle, height of intervertebral space at operative level were recorded. Results The operation time, intraoperative bleeding volume, intraoperative fluoroscopy times in the MRN group were better than those in the conventional group(P<0.001). The changes of JOA value, VAS value and C2～C7 Cobb angle in 6 months and 12 months after operation were significantly improved compared with those before operation(P<0.05). Conclusion Preoperative brachial plexus MRN precise location of cervical disc herniation could reduce the damage to zygapophysial joint and other structures caused by grinding bone window in PPECD, avoid the injury of cervical nerve and dura mater caused by searching for herniated cervical intervertebral disc, reduce intraoperative bleeding, fluoroscopy times and operation time, and obtain the best result with less trauma. Surgical effect, operation safety is higher, patients recover faster after operation.
• Select
  Safety ablation of facet joint in percutaneous posterior endoscopic cervical discectomy

  ZHENG Zhenyang, SHI Qi

  滨州医学院学报. 2020, 43(1): 23-26. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.006

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To study the safety removal range of facet jointsin percutaneous posterior endoscopic cervical discectomy by applied anatomy method, and to provide theoretical basis for clinical surgery. Methods Five adult cadaver specimens were selectedto observe the side block height(a) and width(b) and to observe the inside edge of the intersection (O point) after facet joint space and the horizontal distance after resection facet nerve root only point (c), and nerve root trailing edge of the vertical distance (d), the lower edge of the inner upper pedicle (G point) and the upper edge of the inner lower pedicle (H point) connection horizontal distance (e), and the horizontal distance between the inner side wall of the vertebral artery (f) and removal of the rear facet revealing nerve root length (g). The data obtained were analyzed statistically. Results a was (10.06±0.89~11.39±0.97)mm, b was (10.30 ± 0.89~12.29 ± 0.47)mm, c was (3.99±0.60~5.12±0.44)mm, d was(3.79±0.44~4.57±0.44)mm, e was (1.69±0.87~4.83±0.96)mm, f was (3.88±0.90~6.40±0.79)mm, and g was (8.44±0.87~12.34±0.88)mm. Conclusion In PPECD, the safe and effective range of excision of the facet joint from the O point: the outward range of C_3~4 should not exceed 38% of the corresponding facet joint, and should not exceed 45% in C_5~7. This range can effectively expose the lesion location and reduce tissue damage.
• Select
  Epidemiological study of anterior cruciate ligament injury in trauma population

  DING Zhaoming, CHEN Bing

  滨州医学院学报. 2020, 43(1): 27-29. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.007

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To study the epidemiological characteristics of anterior cruciate ligament(ACL)injury in trauma people. Methods Four hundred and fifteen patientswho received a magnetic resonance examination of the knee in our institute from July 2015 to June 2016 were studied retrospectively, including 240 males and 175 females. The incidence of injury, gender difference and ligament injury were analyzed. Results The incidence of anterior cruciate ligament injured in the trauma population was 20.9%, withmale 22.0% and female 19.4% (P>0.05). The incidence of anterior cruciate ligament injured with other major ligaments was 42.7%, and the incidence of Combined medial collateral ligament was 28.1% (P<0.05). Conclusion The incidence of anterior cruciate ligament injured in the trauma population was 20.9%, showing no gender differences. The medial collateral ligament was most often merged with anterior cruciate ligament injury (28.1%).
• Select
  The relationship between cognitive function and plasma homocysteine level in patients with subcortical ischemic vascular disease

  SONG Xiaowen, SONG Weiwei, ZHENG Haifei, CHEN Jinbo

  滨州医学院学报. 2020, 43(1): 30-33. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.008

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To investigate the relationship between cognitive function and plasma homocysteine (Hcy) level in patients with subcortical ischemic vascular disease (SIVD). Methods Data of SIVD patients were collected (n=130), and these patients were divided into the SVMCI group (n=72) and the SVAD group (n=58). And 60 people with normal cognitive function were selected as the control group (n=60). MMSE, MoCA and CDR were used to evaluate the degree of dementia in the two patient groups. Indicators were compared and analyzed. Results The Hcy concentration in the SIVD group was significantly higher than that in the control group(P<0.05), and the plasma Hcy level in the SIVD group increased significantly with the increase of dementia degree (P<0.05). In the SIVD group, Hcy level was positively correlated with the degree of dementia (P<0.05), MMSE and MOCA score (P<0.05). Conclusion High levels of hcy are associated with the pathogenesis of SIVD, and hyperhcyemia is an important factor in the pathogenesis of SIVD.
• Select
  Detection of LHCGR gene mutation and pedigree analysis in the family with empty follicular syndrome

  WANG Liting, WANG Chengxiang, SUN Hongliang, WANG Yanlin, LI Qingchun

  滨州医学院学报. 2020, 43(1): 34-37. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.009

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To explore the relationship between LHCGR gene mutation and empty follicular syndrome and to guide the diagnosis and treatment of related reproductive diseases. Methods An analysis was carried out on LHCGR gene mutation and pedigree of a infertile patient with empty follicular syndrome by gene sequencing. Results There was homozygous mutation in exon 11 of LHCGR gene in this patient: chr2:48915113A>G, c.1823T>C, p. Leu608Pro. The mutation resulted in the mutation of LHCGR protein from leucine (Leucine, Leu) to proline (Proline, Pro) at position 608. The family analysis of the patient and sequencing of the family members showed that the parents belonged to the heterozygous mutation, the proband brother (46, XY) was a homozygous mutation, and the proband sister (with a girl and a boy) belonged to a heterozygous mutation. Conclusion LHCGR gene mutation can lead to empty follicle syndrome, which in turn leads to the occurrence of related reproductive diseases.
• Select
  Application of dualsource CT imaging in diagnosis of anterior cruciate ligament injury

  REN Rui, SONG Qiong, ZHANG Zhilong, CHEN Liang, ZHAI Changbin

  滨州医学院学报. 2020, 43(1): 38-41. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.010

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To evaluate the diagnostic accuracy of dual-source CT imaging in anterior cruciate ligament injury of knee joint by comparing the results of arthroscopy with anatomy and dual-source CT imaging. Methods Thirty-six patients with unilateral anterior cruciate ligament injury were treated with dual-source CT imaging.The original image is processed into MPR, RT and dual-energy images through post-processing software. CT density, length and starting point, middle and terminal ligament thickness of bilateral anterior cruciate ligament (ACL) were measured to locate the injury site of ACL. Arthroscopic examination was performed on the affected knee joints in 36 patients.The measurement values between the affected side and the normal side of the anterior cruciate ligament were measured by independent sample t test, with P<0.05, and the difference was statistically significant.Kappa analysis was used for the consistency of the two tests. Kappa values<0.20 were considered to be very low consistency, 0.21 to 0.40 general consistency, 0.41 to 0.60 medium consistency, 0.61 to 0.80 height consistency, and 0.81 to 1 almost identical. Results Thirty patients with unilateral anterior cruciate ligament injury were diagnosed by arthroscopy, and the coincidence rate, true positive rate, missed diagnosis rate, true negative rate and misdiagnosis rate of dual-source CT were 91.67%, 93.33%, 6.67%, 83.33% and 16.67%, respectively. The CT density, length, starting-point thickness, middle thickness and terminal thickness of the anterior cruciate ligament on the affected side (patients with ACL injury diagnosed by arthroscopy) were significantly different from those on the healthy side(P<0.05).Kappa test was used for arthroscopy and dual-source CT examination, with Kappa value of 0.719, and the two methods had consistency. Conclusion Dual-source CT imaging technology can be used as an important imaging auxiliary examination in the diagnosis of anterior cruciate ligament injury. Dual-source CT can preliminarily diagnose and locate the injury site of anterior cruciate ligament of knee joint by measuring the anterior cruciate ligament related imaging.
• Select
  The value of ultrasonography in detection of bypass grafts after ligmentum teres hepatis-bridged combined with portacaval shunt

  ZHANG Fengqin, XU Yongbo, XU Cui, WANG Kun, CUI Guanghe

  滨州医学院学报. 2020, 43(1): 42-45. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.011

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To explore the value of ultrasonography in detection of bypass grafts after Ligmentum teres hepatis-bridged combined with portacaval Shunt. Methods There were 43 patients of bypass grafts after Ligmentum teres hepatis-bridged combined with portacaval Shunt. Two-dimensional ultrasound and color Doppler was used to observed the bypass grafts at 3, 12 and 24 months respectively after the operation. According to different ultrasonic manifestations of bypass grafts, 2 groups were divided: group of patency of bridging vessels and group of Bridge stenosis. Results Three months after surgery, there were 41 cases with vascular patency, and 2 cases with vascular stenosis; twelve months after surgery, there were 38 cases with vascular patency, and 5 cases with vascular stenosis; Twenty-four months after surgery, there were 30 cases with vascular patency, and 13 cases with vascular stenosis. The probability of stenosis of the bypass grafts increased with time, while 3 months after surgery was compared with 24 months after surgery, P<0.05. Conclusion The color Doppler ultrasound was used as an important method to evaluate the success of the operation.
• Select
  Evaluation of shear wave elastography in differentiation diagnosis of thyroid nodules using logistic regression

  XU Yongbo, YU Yanjie, SUN Jian, SUN Fang, YANG Zhi, ZHANG Wenxiao

  滨州医学院学报. 2020, 43(1): 46-48. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.012

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To evaluate the value of conventional ultrasonic and shear wave elastography in the diagnosis of thyroid nodules by applying the binary Logistic regression model. Methods Totally 239 cases of thyroid solitary nodules were examined by conventional ultrasonic and shear wave elastography.A Logistic model was obtained on the basis of ultrasonographic features. A receiver operator characteris(ROC) curve was constructed to assess the performance of the Logistic model. The differential diagnosis value of shear wave elastography in thyroid nodules was evaluated. Results Four ultrasonographic features including boundary, calcification, Emax and Emean finally entered into the Logistic model. The percentage of correct prediction, the sensitivity and specificity were 91.60％, 92.90% and 89.90%. Conclusion Shear wave elastography plays important role in the differential diagnosis of thyroid nodules.
• Select
  Clinical study of four different methods of bone augmentation technology in dental implant

  ZHANG Bin, ZHANG Junhua, SUN Lihua, LIU Yusan, LI Yourui

  滨州医学院学报. 2020, 43(1): 49-51. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.013

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To evaluate the four different methods of bone augmentation technology in dental implant. Methods Seventy-nine cases of dental defects were selected, including the guided bone regeneration (GBR) 27 cases, 12 cases of onlay bone grafting, 23 cases of intra-maxillary sinus elevation, 17 cases of extra-maxillary sinus elevation). Six months after the implantation, the horizontal transfer mold was used to make zirconia all-ceramic prosthesis. One, two and three years after the repair, the patients were examined again to observe the health of the gingival tissue around the implant and the use of all-ceramic crown, the bone tissue absorption around the implant and subjective satisfaction of patients with implant denture. Results Before full crown restoration, one implant in GBR group became loose and fell off due to early contact with the invisible denture. After full crown repair, 2 base table screws in intra-maxillary sinus elevation group were loose. In group of extra-maxillary sinus elevation, 1 crown porcelain fell off, and the rest of the implants had good stability. The onlay group had more bone resorption in the first year, P＜0.05. There was no statistical difference in bone resorption between the four groups in the second and third years, and all obtained good soft and hard tissue effect. Conclusion Four different bone augmentation methods can achieve good clinical results.
• Select
  Clinical study on the effects of different oral rehabilitation membranes in guided bone regeneration of dental implantation

  WANG Xiaoli, HOU Yudong

  滨州医学院学报. 2020, 43(1): 52-53. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.014

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To evaluate the clinical effects of different oral rehabilitation membranes in guided bone regeneration (GBR) of dental implantation. Methods Ninty patients were selected which underwent dental implantation GBR. They were randomly divided into two groups. The Experimental Group (n=45) was treated with HEAL-ALL dental rehabilitation membrane, while the Control Group (n=45) was treated with titanium membrane. The postoperative therapeutic effects, bone density, thickness and the occurrence of complications were compared between two groups. Results The postoperative therapeutic effects of the experimental group was better than that of the control group, (P<0.05). There was statistical significance on bone density and thickness of bone graft between two groups (P＜0.05). The occurrence rate of postoperative complications of HEAL-ALL group was significantly lower than that of titanium group (P＜0.05). Conclusion The HEAL-ALL membranes were beneficial to the bone regeneration of dental implantation. The postoperative therapeutic effects were significant and the occurrence rate of complications was very low, so it is suitable for clinical application.
• Select
  Development of HPLC method of simultaneous determination of baicalin, baicalein and wogonin from Scutellariae Radix extracts

  LIU Qingqing, WANG Yubo, LIU Jianwei, XU Qianqian, ZHANG Ranran, WANG Yiqing

  滨州医学院学报. 2020, 43(1): 54-57. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.015

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To develop the high performance liquid chromatography (HPLC) method for simultaneous determination of baicalin, baicalein and wogonin from different extracts of Scutellariae Radix. Methods Alcohol solutions of different concentration and distilled water were used as extract solutions, decompression concentration was use to prepare the extract. Specialty validation, linearity determination, precision determination and stability valuation were used to determine the reliability of method. The established HPLC-UV method was used to determine contents of baicalin, baicalein and wogonin in extractions. Results A HPLC method was established with Agilent Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm). Mobile phase was acetonitrile-0.05% phosphate acid solution at a flow rate of 1.00 mL/min. The UV detection wavelength was 275 nm. The injection volumn was 10 μL. The column temperature was 30 ℃. The contents of baicalin, baicalein and wogonin in different Scutellariae Radix extractions were finally detected. Baicalin, baicalein and wogonin showed good relationship in the linear range of 0.97～19.45, 1.71～34.10, 0.61～12.25 μg/mL, respectively. Relative standard deviation (RSD) of precision, repeatability and stability were all less than 2.0%. Conclusion HPLC has high sensitivity, high precision, and good specificity and is suitable for the determination of baicalin, baicalein and wogonin in different extractions of Scutellariae Radix.
• Select
  Monitoring of perioperative sleep status of patients undergoing hepatobiliary surgery for accelerated rehabilitation

  YANG Zhen, FANG Xiuxin, LYU Xiaoqin, WU Qianqian

  滨州医学院学报. 2020, 43(1): 58-61. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.016

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To understand the perioperative sleep status of patients undergoing accelerated rehabilitation hepatobiliary surgery, and provide theoretical basis for the further development of sleep management strategies. Methods Two Hundred cases of patients undergoing accelerated rehabilitation surgery were selected as the study subjects. Sleep monitoring bracelet was used to dynamically monitor the sleep status of perioperative patients. Results The incidence of perioperative sleep insufficiency in patients with accelerated hepatobiliary surgery reached up to 73%. Perioperative sleep of patients with pancreatic surgery was the worst. Perioperative sleep duration of open surgery was lower than that of laparoscopic surgery (P<0.05). The perioperative sleep duration of patients undergoing grade Ⅳ surgery was lower than that of patients undergoing grade Ⅲ surgery (P<0.05). Conclusion The perioperative sleep problem of patients in hepatobiliary surgery was not optimistic. There were differences in perioperative sleep status among patients with different operation types, surgical methods and surgical grades. Medical staff should pay great importance to the sleep status of perioperative patients, further explore the influencing factors of sleep problems, and take active and effective nursing intervention measures.
• Select
  The research progress of daglicaten on chronic complications of diabetes

  DING Hualin, YU Suguo

  滨州医学院学报. 2020, 43(1): 62-64. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.017

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Sodium-glucose cotransporter 2 (SGLT-2) is a key protein molecule involved in glucose reabsorption in the renal tubules, while SGLT-2 expressed in diabetic patients is higher than that in normal people. Therefore, SGLT-2 is a treatment for diabetes and concurrency in recent years. The new target of the disease, its unique mechanism of action has become another innovative study in the field of diabetes. The SGLT-2 inhibitor (SGLT-2i) developed for this target is a non-insulin-dependent new oral hypoglycemic drug that can reduce the reabsorption of glucose by the kidneys and increase the excretion of urine sugar to reduce the effect of blood sugar. Among them, dapagliflozin is a highly active and highly selective SGLT-2i. This article focuses on the research progress of dapagliflozin in improving diabetes and its chronic complications.
• Select
  Advances in research on the mechanism of action of resveratrol against pituitary prolactinoma

  WANG Bo

  滨州医学院学报. 2020, 43(1): 65-67. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.018

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Prolactin-type pituitary adenoma is the highest clinically occurring pituitary tumor. Hyperprolactinemia is caused by excessive secretion of pituitary prolactin by pituitary prolactin cells, which leads to impotence infertility in male patients or menstrual disorders or infertility in female patients. At present, oral dopamine receptor agonists such as bromocriptine or cabergoline are the first choice for clinical treatment. These drugs directly affect the pituitary gland to inhibit the secretion of prolactin, thereby reducing the blood prolactin level. Approximately one-quarter of patients who remained on oral medication did not return to normal levels. Resveratrol is a polyphenolic compound extracted from plants. It is widely present in plants such as Polygonum cuspidatum and grapes, and has a variety of biological activities in the literature, such as phytoestrogens, antitumor, and cardiovascular protection. This article reviews the mechanism of resveratrol against pituitary prolactinoma and provides a new idea for the treatment of prolactin pituitary tumor.
• Select
  Investigation on the current situation of cross-specialty learning attitude of on-the-job postgraduate students in nursing and analysis of influencing factors

  YUAN Shuping, ZHANG Min, DU Xiuyan, ZHAO Xueying, GAO Yunxiu, LI Ming'e

  滨州医学院学报. 2020, 43(1): 68-71. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.019

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To understand the cross-disciplinary learning attitude and influencing factors of on-the-job nursing postgraduates, and to provide reference for the development of cross-disciplinary education among on-the-job nursing postgraduates. Methods The general information questionnaire and the Chinese version of the preparation for cross professional cooperative learning questionnaire were used to investigate on-the-job nursing postgraduates in Shandong Province. Results The total score of cross-disciplinary cooperative learning readiness scale for on-the-job nursing postgraduates was (81.74±7.63), which was at a high level. According to the classification of professional titles, there were statistically significant differences (P<0.05) in the scores of cross-disciplinary cooperative learning readiness scale for on-the-job nursing postgraduates with different professional titles. According to the classification of departments, there were statistically significant differences in the scores of cross-disciplinary cooperative learning readiness scale for on-the-job nursing students in different departments (P<0.05). Conclusion Cross major learning attitude of on-the-job nursing postgraduates is good, they have high team spirit and professional identity, and can correct understanding of their roles and responsibilities. Nursing management and educators should pay attention to the importance of cross major learning, constantly improve professional collaboration ability of on-the-job nursing postgraduates, thus improve the quality of nursing service level, and provide better service for patients.
• Select
  Application of PBL combined with standardized patients in clinical teaching of nephrology

  ZHANG Xiaomin, DONG Hua, SUN Xiaotong, LIU Yunqi, GAO Jie

  滨州医学院学报. 2020, 43(1): 72-74. https://doi.org/10.19739/j.cnki.issn1001-9510.2020.01.020

  Abstract ( ) Download PDF ( )   Knowledge map   Save

  Objective To evaluatethe application of PBL combined with standardized patients in clinical teaching of nephrology. Methods Totally 270 fourth grade medical students of five-year clinical medicine specialty in one medical university were divided into three groups: the PBL group, the PBL+SP group and the traditional group. Questionnaires and assessment wereperformed at the end of the course. Results The results ofquestionnaire showed 100% of the students in the PBL+SP group were satisfied or very satisfied with the course, 98.51% of the students in the PBL group were satisfied or very satisfied with the course, while only 80.74% of the students in the traditional group were satisfied the course. The satisfaction rate of the PBL+SP group and the PBL group were significantly higher than that of the traditional group (P<0.05) and the percentage of students very satisfied withthemethodin PBL+SP group was significantly higher than that of the PBL group (P<0.05). Students in the PBL+SP group were willing to apply with SP in the future. The assessment showed that the score of PBL+SP group and PBL group were significantly higher than that of the traditional group in learning interest, initiative, creativity, clinical practice ability, comprehensive communication skills with patients, teamwork and critical thinking (P<0.05).And the score of the PBL+SP group was significantly higher than that of thePBL group in learning interest,clinical practice ability, critical thinking, comprehensive communication skills with patients, expressive language skills (P<0.05). The collecting medical history and theoretical knowledge scores of PBL+SP group and PBL group were significantly higher than that of the traditional group students (P<0.05), and the collecting medical history score of PBL+SP group was significantly higher than PBL group (P<0.05). Conclusion The application of PBL combined with SP in clinical teaching of nephrology will enhance students' interest, initiative, creativity and clinical practice ability, develop students' comprehensive communication skills with patients, teamwork and critical thinking, and is worth promoting in clinical teaching.