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| The effect of chlorimipramine on the spontaneous contraction of isolated duodenal smooth muscle of rabbits |
| WANG Lei1, XIA Shuai1, GONG Yushuang1, WANG Na2, SUN Yuqi1, JIANG Shujun2 |
1 Clinical College,Binzhou Medical University,Yantai 264003,P.R.China;
2 Department of Physiology,Basic Medicine College,Binzhou Medical University |
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Abstract Objective To investigate the effect of chlorimipramine (CIMP) on the spontaneous contraction of duodenal smooth muscle of rabbits in vitro and its mechanism.Methods The isolated intestinal tubes of rabbits were made.The technique of perfusion of isolated duodenum was used to record duodenal smooth muscle contraction curve.The influence of CIMP on the spontaneous contraction of duodenal smooth muscle was observed and the mechanism of CIMP was studied with pharmacological methods.Results CIMP reduced the amplitude of spontaneous contraction of duodenal smooth muscle in a dose-dependent manner,while the frequency was not obviously influenced.CIMP inhibited the enhanced contraction of duodenum smooth muscle induced by acetylcholine.CIMP also inhibited the contraction of duodenum smooth muscle induced by calcium chloride application in the calcium-free Tyrode’s solution.ACh and CaCl2 were added successively into calcium-free Tyrode’s solution,and CIMP was not effective on the release of internal calcium, but inhibited external calcium flow in the dose-dependent manner.Conclusion CIMP obviously inhibits the spontaneous contraction of duodenal smooth muscle of rabbits in vitro. Suppression of M receptor and inhibition of the extracellular calcium influx via cell membrane might be involved in the process.
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Received: 21 November 2017
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