肾细胞癌外周血中长链非编码RNA差异表达分析

doi:10.19739/j.cnki.issn1001-9510.2021.04.005

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Abstract Objective To investigate the expression of long noncoding RNA (lncRNA) and mRNA in peripheral blood of renal cell cancer (RCC).Methods Five RCC patients diagnosed by pathology were selected as the case group, and the other five healthy persons who were routinely examined in the outpatient clinic were selected as the control group. All the cases were extracted the peripheral blood and the expression of lncRNA and mRNA were detected by gene chip technology. Two upregulated lncRNA ENST00000547549, ENST00000591689 and one down-regulated lncRNA NR_038263 were selected randomly for verification analysis by real-time fluorescence quantitative PCR.Results Compared with those in the control group, 3 664 lncRNA were differentially expressed in the peripheral blood, of which 1 511 lncRNA were up-regulated, 2 153 lncRNA were down-regulated (fold change≥2, P<0.05). And 2 268 mRNA were differentially expressed, of which 932 mRNA were up-regulated, and 1 336 mRNA were down-regulated (fold change≥2, P<0.05). The verification analysis of the three lncRNA ENST00000547549, ENST00000591689 and NR_038263 by real-time quantitative PCR, and the results were consistent with the results of the chip detection.Conclusion The lncRNA gene chip is a high-efficiency and rapid detection method for detecting differentially expressed genes in peripheral blood of RCC. The differentially expressed lncRNA and mRNAs in peripheral blood may be potential biomolecules for the diagnosis and follow-up of RCC, and may become therapy targets.

Key words： renal cell carcinoma lncRNA peripheral blood expression difference

Received: 16 March 2021

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	Articles by authors
	GONG Haifeng
	CHU Wei
	ZHANG Bing
	ZHENG Jing
	SU Qingguo
	MEI Yanhui
	JI Hong

Cite this article:

GONG Haifeng,CHU Wei,ZHANG Bing, et al. Different expression of lncRNA in peripheral blood of renal cell carcinoma[J]. 滨州医学院学报, 2021, 44(4): 261-265.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.04.005 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I4/261

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