寨卡病毒非结构蛋白1对小鼠免疫应答的影响

doi:10.19739/j.cnki.issn1001-9510.2021.02.001

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Abstract Objective To use Zika virus nonstructural protein 1 (NS1) expressed in prokaryotic system, to observe the effect of NS1 on immune response of mice, so as to explore the potential of NS1 as Zika virus vaccine.Methods The recombinant NS1 protein was obtained by expression pET28a-ZIKV-NS1 plasmid in prokaryotic expression system, then the protein was be used to immunize the mice. The immunoglobulin G (IgG) in mouse serum was determined by means of enzyme-linked immunosorbent assay. And the flow cytometry and enzyme-linked immune assay were applied to detecting related factors including the mice spleen's CD4⁺ cells, CD8⁺ cells numbers and cytokine interferon gamma (IFN-γ), interleukin 4 (IL-4) secretion levels.Results We constructed the prokaryotic expression plasmid pET28a- ZIKV-NS1 successfully and got the ZIKV-NS1 protein. The serum IgG titer of mice increased when NS1 was immunized 2 and 3 times, and increased with the increase of inoculation times, and when compared with the blank control group, P<0.001; the number of CD4⁺ and CD8⁺ cells as well as the level of IFN-γ and IL-4 secretion in the spleen of mice increased, and when compared with the blank control group, P<0.001.Conclusion ZIKV-NS1 can activate the humoral and cellular immunity of mice, which may have the potential to become a Zika virus vaccine.

Key words： Zika virus nonstructural protein 1 CD4⁺cell CD8⁺ cell interferon-γ interleukin-4

Received: 04 January 2021

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R34

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	Articles by authors
	RONG Xiaorong
	YANG Yanpeng
	ZHAO Dan
	WANG Yuanfang

Cite this article:

RONG Xiaorong,YANG Yanpeng,ZHAO Dan, et al. Effects of zika virus NS1 on immune response in mice[J]. 滨州医学院学报, 2021, 44(2): 81-87.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.02.001 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I2/81

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