浓缩生长因子提取液对牙龈间充质干细胞增殖及成骨分化的促进作用

doi:10.19739/j.cnki.issn1001-9510.2020.04.017

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Abstract Objective To evaluate the effect of concentrated growth factors extract (CGFe) on the proliferation of gingiva-derived mesenchymal stem cells (GMSCs) and differentiation of F-actin cytoskeleton.Method GMSCs were cultured in vitro.Cells in the experimental group were cultured with α-MEM containing CGFe (10% FBS),while cells in the control group were cultured with α-MEM without CGFe (10% FBS).The proliferation of GMSCs cells was determined by MTT assay,the activity of alkaline phosphatase (ALP) was detected at 1,3,5 and 7 d after osteogenesis induction,the formation of calcium nodules was detected by alizarin red staining at 14 and 21 d,and the morphology of the F- actin cytoskeleton was observed by confocal laser scanning microscope (CLSM).Results A significant increase of absorbance at 1,3 and 5 d was showed in the experiment group compared to that in the control group,P＜0.05.On the assessment of ALP activity of GMSCs,the absorbance of the experiment group at 1,3 ,5 and 7 d was significantly higher than that of the control group,P<0.05 or P<0.01.On the assessment of osteogenic differentiation activity,the mineralized nodules were detected by the alizarin red (Sigma) staining.The overall absorbance of the calcium nodules in the experimental group at 7,14 d was significantly higher than that in the control group,P<0.01.Conclusion CGFe can significantly promote the proliferation of GMSCsand osteogenic differentiation.

Key words： concentrated growth factor extract gingiva-derived mesenchymal stem cell cell proliferation osteogenicdifferentiation

Received: 16 March 2020

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R329.2

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	Articles by authors
	ZHANG Rimei
	CAI Miao
	LI Zhenjie
	HAO Pengjie

Cite this article:

ZHANG Rimei,CAI Miao,LI Zhenjie, et al. Promotion effect of concentrated growth factor extracton on the proliferation and differentiationof gingiva-derived mesenchymal stem cells[J]. 滨州医学院学报, 2020, 43(4): 297-300.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.04.017 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I4/297

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