肝硬化大鼠ALPPS术后肝功能及肝再生的变化

doi:10.19739/j.cnki.issn1001-9510.2020.02.003

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Abstract Objective To test serum levels of ALT,AST,ICG R15 and calculating the hepatic regeneration rate (HRR) of remnants (median lobe) on normal and cirrhosis rats,to explore the rule of the liver function and regeneration after operation and analyse the correlation. Methods Thirty male SD rats with normal and cirrhosis (induced by CCl₄) were randomly selected. The two group rats were divided into 5 subgroups, including the 1st, 3rd, seveth, tenth, and fourteenth day subgroups. All of the rats were underwent ALPPS and were tested AST、ALT and ICG R15, and regeneration rate of residual liver was count. Results AST, ALT of two groups were raised after surgery, the experimental group was obviously higher than the control group, which had statistical difference. Next to the two levels were decreased gradually, the control group was normal in 7 days after surgery, while the experimental group was normal in 14 days after surgery. ICG R15 had the same change. Regeneration rate of residual liver increase gradually, but regeneration rate of the control group was faster than the experimental group. The correlation analysis showed thatthey had obvious correlation (r=0.841). The single factor linear regression analysis obtained an equation of regression: the control group was Y= -0.1647x+39.396 (X was ICG R1 5, Y was regeneration rate of residual live). Conclusion The liver function injury of the experimental group was more obvious than the control group, and the recovery of liver function was slower than the control group, too. And the liver function had correlation with liver regeneration. So the volumes of residual live could be used as an index for evaluating liver function.

Key words： liver partition portal vein ligation liver regeneration liver function Hepatic cirrhosis

Received: 06 May 2019

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R363

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	Articles by authors
	QIN Yugang
	ZHANG Qiang
	ZHAO Zhe
	YI Wen
	BAI Tianyi
	WEI Xiaojun
	OUYANG Caiguo

Cite this article:

QIN Yugang,ZHANG Qiang,ZHAO Zhe, et al. Change of liver function and regeneration after portal vein ligation dombined with in situ splitting on in cirrhosis rats[J]. 滨州医学院学报, 2020, 43(2): 92-96.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.02.003 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I2/92

[1] 董家鸿,郑树森,陈孝平,等.肝切除术前肝脏储备功能评估的专家共识(2011版)[J].中华消化外科杂志,2011,10(1):20-25.
[2] LIU H, ZHU S. Present status and future perspectives of preoperatire portal vein embolization[J].Am J Surg,2009,197(5):686-690.
[3] SHINDOH J, VAUTHEY J N, ZIMMITTI G, et al. Anaysis of the efficacy of portal vein embolization for patients with extensive liver malignancy and very low future liver remnant volume, including a comparison with the associating liver partition with portal vein ligation for staged hepatectomy approach[J]. J Am Call Surg,2013,217(1):126-133.
[4] SCHNITZBAUER A A, LANG S A, GOESSMANN H, et al. Right portal vein ligation combined with in situ splitting induces rapid left lateral liver lobe hypertrophy enabling 2-staged extended right hepatic resection in small-for-size settings [J].Annals of Surgery,2012,255(3):405-414.
[5] 徐大华,孙家邦,李非,等.肝硬化时人工气腹对肝脏血流的影响——大鼠肝硬化模型吲哚青绿排泄实验研究[J].中华普通外科杂志,2001,16(12):762。
[6] 顾向前,郑虹,曹顺琪,等.联合肝脏离断和门静脉结扎术后大鼠肝再生的实验研究[J].中华肝胆外科杂志,2016,22(6):412-416.
[7] 刘红梅,张玉玲,康金玲,等.复合因素诱导大鼠肝硬化模型的建立[J].宁夏医科大学学报,2012,34(3):310-312.
[8] YAO L, LI C, GE X, et al. Establishment of a rat model of portal vein ligation combined with in situ splitting[J].PLos one,2014,9(8):e105511.
[9] LIN C L, KAO J H. Risk stratification for hepatitis B virus related hepatocellular carcinoma[J].J Gastroenterol Hepatol,2013,28 (1):10-17.
[10] MAKUUCHI M, THAI B L,TAKAYASU K, et al. Preoperative portal embolization to increase safety of major hepatectomy for hilar bile duct carcinoma: a preliminary report[J].Surgery,1990,107(5):521-527.
[11] KOKUDO N, TADA K, SEKI M, et a1.Proliferative activity of intrahepatic colorectal metastases after preoperative hemihepatic portal vein embolization [J]. Hepatology,2001,34(2):267-272.
[12] HAYASHI S, BABA Y, UENO K, et al. Acceleration of primary liver tumor growth rate in embolized hepatic lobe after portal vein embolization [J]. Acta Radiol,2007,48(7):721-727.
[13] 史冀华,朱盛兴,张水军.大鼠肝部分切除术的应用解剖及实施[J].世界华人消化杂志,2008,16(22):2516-2520.
[14] FAN S T. Liver functional reserve estimadon:state of the art and relevance for local treatments:the Eastern perspective[J]. Hepato Biliarv Pancreatic Sciences,2010,17 (4):380-384.
[15] 林栋栋,卢实春,李宁.小肝移植物与小肝综合征[J].首都医科大学学报,2011,32(3):356-360.
[16] LU X, ZHAO H, YANG H, et al. A prospective clinical study on early recurrence of hepatocellular carcinoma after hepatectomy [J]. J Surg Oneo,2009,100(6):488-493.