静脉注射人胎盘间充质干细胞对油酸型急性肺损伤炎症调节的作用

doi:10.19739/j.cnki.issn1001-9510.2020.03.003

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Abstract Objective To investigate the therapeutic effect of human placenta mesenchymal stem cells (hPMSCs) on OA-induced acute lung injury and its possible mechanism.Methods Thirty-six SD rats were divided into the control group, the lung injury group and the treatment group. Acute lung injury rat model was prepared by sublingual intravenous injection of oleic acid. After the successful modeling, the samples were kept for 24 h after the corresponding treatment, and the lung tissue morphology, the ratio of wet to dry mass of lung tissue (W/D), the proportion of neutrophils in alveolar lavage fluid and the serum IL-10, IL-8 level were detected.Results Pulmonary histopathology showed that normal alveolar structure was normal in the normal group, typical ALI changes were observed in the lung tissue of the lung injury group, and the degree of injury in the cell transplantation group was relatively mild. Compared with the control group, the number of neutrophils in bronchoalveolar lavage fluid was significantly increased in the injury group and the transplantation group (P<0.05 or <0.01), and the wet/dry ratio of bronchoalveolar lavage solution was also increased in the injury group and the transplantation group (P<0.05). Compared with the lung injury group, the number of neutrophils and the wet/dry ratio of bronchoalveolar lavage fluid in the transplantation group were all decreased (P<0.05). Plasma and lung tissue levels of IL-8 and IL-10 in the injury group and the transplantation group were significantly increased, compared with the control group (P<0.05 or <0.01); IL-8 levels in the transplant group were significantly reduced compared with the injury group (P<0.05 or <0.01).Conclusion Placental mesenchymal stem cell transplantation in the treatment of acute lung injury can reduce the level of inflammatory factors, improve the level of anti-inflammatory factors, stabilize the internal environment, avoid the occurrence of inflammatory storms, and provide an effective treatment method for the clinical treatment of critical patients.

Key words： acute lung injury mesenchymal stem cell IL-10 IL-8 cell transplantation

Received: 28 September 2019

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R563.1

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	Articles by authors
	CUI Qingqing
	XING Chaopin
	LIN Xinmei
	ZHANG Xinshun
	DUAN Peifeng
	LI Gaofeng

Cite this article:

CUI Qingqing,XING Chaopin,LIN Xinmei, et al. Regulatory effects of human placenta derived mesenchymal stem cells on the treatment for OA-induced acute lung injury[J]. 滨州医学院学报, 2020, 43(3): 169-171.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.03.003 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I3/169

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