张应力作用下小鼠脂肪间充质干细胞骨向分化过程中成骨相关微小RNA表达模式

doi:10.19739/j.cnki.issn1001-9510.2021.04.002

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Abstract Objective To investigate the osteogenesis-related microRNA (miRNA) expression pattern during osteogenic differentiation of murine adipose-derived mesenchymal stem cells (ADSCs) under tensile stress.Methods ADSCs of the third generation of mice were divided into two groups, the experiment and the control group. The experiment group cells were subjected to a cyclic uniaxial tensile strain generated by a four-point bending apparatus, while the control group cells were without tension treatment. QRT-PCR (quantitative real-time PCR) was used to detect the expression of OCN (Osteocalcin), ALP (alkaline phosphatase), Col1 (type I collagen) and Runx2 (runt-related transcription factor 2) mRNA at different time points. Microarray was used to screen osteogenesis-related miRNA,and the relative expression intensity of miRNAs was detected during the application of tension.Results The mRNA levels of osteogenic genes in the experimental group were relatively high. Five up-regulated and 6 down-regulated miRNAs were screened out. On the third day of tensile stress stimulation, all miRNAs showed different changes, and most miRNAs showed a continuous up-regulation or down-regulation trend in the process of augmentation, with the most significant difference on the fifth day.Conclusion Tensile stress can induce the osteogenic differentiation of the ADSCs, which may be attributed to the osteogenesis-related miRNA.

Key words： adipose-derived mesenchymal stem cell tensile stress microRNA microarray osteogenic differentiation

Received: 28 March 2021

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	LI Jianwei
	LI Huanhuan
	YANG Yong
	ZHANG Pei
	HUANG Yumei

Cite this article:

LI Jianwei,LI Huanhuan,YANG Yong, et al. Osteogenesis-related microRNA expression pattern analysis during osteogenic differentiation of murine adipose-derived mesenchymal stem cells under tensile stress[J]. 滨州医学院学报, 2021, 44(4): 247-251.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.04.002 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I4/247

[1] DOLLY M, ANDREAS S, CORNELIA K, et al. Isolation, cultivation, and characterization of human mesenchymal stem cells[J]. Cytometry A, 2018, 93(1):19-31.
[2] HONG P, YANG H, WU Y, et al. The functions and clinical application potential of exosomes derived from adipose mesenchymal stem cells: a comprehensive review[J]. Stem Cell Res Ther, 2019, 10(1):242.
[3] SHAFAEI H, KALARESTAGHI H. Adipose-derived stem cells: an appropriate selection for osteogenic differentiation [J]. J Cell Physiol, 2020, 235(11):8371-8386.
[4] DU H M, ZHENG X H, WANG LY, et al. The osteogenic response of undifferentiated human adipose-derived stem cells under mechanical stimulation[J]. Cells Tissues Organs, 2012, 196(4):313-324.
[5] DAVIDE C, ANGELA R, MICHELE C, et al. MicroRNA-mediated regulatory circuits: outlook and perspectives[J]. Phys Biol, 2017, 14(4):045001.
[6] ROSTAMI Z, KHORASHADIZADEH M, NASERI M. Immunoregulatory properties of mesenchymal stem cells: Micro-RNAs[J]. Immunol Lett, 2020, 219(1):34-45.
[7] BINBIN L I. MicroRNA regulation in osteogenic and adipogenic differentiation of bone mesenchymal stem cells and its application in bone regeneration[J]. Curr Stem Cell Res Ther, 2018, 13(1):26-30.
[8] HUANG C, GENG J, JIANG S. MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells[J]. Cell Tissue Res, 2017, 368(2):229-238.
[9] PENG S, GAO D, GAO C, et al. MicroRNAs regulate signaling pathways in osteogenic differentiation of mesenchymal stem cells[J]. Mol Med Rep, 2016, 14(1):623-629.
[10] SHI C, YANG B, SUN H. New strategies for the prevention and treatment of bone loss - from mechanical loading point of view [J]. Curr Pharm Des, 2017, 23(41):6264-6271.
[11] BAUDEQUIN T, LEGALLAIS C, BEDOUI F. In vitro bone cell response to tensile mechanical solicitations: is there an optimal protocol [J]. Biotechnol J, 2019, 14(1):e1800358.
[12] ZHANG L, WANG Y, ZHOU N, et al. Cyclic tensile stress promotes osteogenic differentiation of adipose stem cells via ERK and p38 pathways[J]. Stem Cell Res, 2019, 37:101433.