地塞米松联合姜黄素对RAW264.7细胞炎症模型的作用

doi:10.19739/j.cnki.issn1001-9510.2021.01.002

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Abstract Objective To explore the anti-inflammatory effect of dexamethasone (DEX) combined with curcumin (CUR) on RAW264.7 cells induced by lipopolysaccharide.Methods The Griess colorimetric method was used to investigate the effect of DEX and CUR alone and in combination in inhibiting NO secretion. The Chou-Talalay combined index method was used to judge the drug interactions, and screen the synergistic anti-inflammatory ratio of the two drugs. Rhodamine-123 efflux experiment was used to clarify the reversal effect of CUR on DEX multidrug resistance (MDR) under a synergistic compatibility ratio. DEX and CUR were administered according to the screened synergistic anti-inflammatory ratio, DEX and CUR co-loaded nanoparticles (DEX/CUR-NPs) were prepared by emulsification solvent evaporation method, and their pharmaceutical properties and anti-inflammatory activity in vitro were investigated. Results When the compatibility molar ratio of DEX and CUR was 1∶5, the CI of each dose was less than 1, showing a synergistic anti-inflammatory effect, and CUR can inhibit the MDR phenomenon of DEX at this ratio. DEX/CUR-NPs was prepared according to the molar ratio of DEX and CUR with 1∶5. The particle size of the DEX/CUR-NPs was (145.5±2.1) nm, the zeta potential was (-20.54±0.98) mV, and the entrapment efficiency of DEX and CUR were (78.9±5.6)% and (96.4±2.4)%, respectively. DEX/CUR-NPs had good sustained-release properties and synergistic anti-inflammatory activity in vitro, and reverse the MDR of DEX.Conclusion DEX and CUR can play a good synergistic anti-inflammatory effect under the compatibility ratio of 1∶5. DEX combined with CUR is a potential candidate combination of anti-inflammatory drugs.

Key words： dexamethasone curcumin inflammation Chou-Talalay joint index method

Received: 23 October 2020

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R965

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	Articles by authors
	ZHANG Qi
	TIAN Baocheng
	ZHAO Qingpei
	SHENG Yuhui
	HUANG Jinghua
	LVQingzhi
	LI Keke

Cite this article:

ZHANG Qi,TIAN Baocheng,ZHAO Qingpei, et al. Effect of dexamethasone combined with curcumin on RAW264.7 cell model[J]. 滨州医学院学报, 2021, 44(1): 7-13.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.01.002 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I1/7

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