HU-308与nHA/PCL电纺丝膜对小鼠前成骨细胞生物学性能的影响

doi:10.19739/j.cnki.issn1001-9510.2020.06.011

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Abstract Objective To explore the effects of different concentrations of HU-308 and nHA/PCL electrospun membranes on the biological properties of mouse pre-osteoblasts (MC3T3-E1).Methods The round electrospun membranes were placed at the bottom of the cell culture plates. MC3T3-E1 osteoblasts were inoculated and cultured in different concentrations of HU-308 (0, 10^-10, 10^-9, 10^-8, 10^-7, 10^-6 mol / L). At 1, 4, and 7 days, cell proliferation and alkaline phosphatase (ALP) activity was detected; RT-qPCR was used to detect osteoblast-related genes OPG mRNA after cultured cells for 7 days. The morphology of the cytoskeleton after 24h was observed.Results After incubation for 1, 4, and 7 days, compared with that in the control group, the proliferation activity of cells in each experimental group was enhanced, P<0.05; with the extension of incubation time, the proliferative activity of cells in the same experimental group increased gradually, P<0.05. With the extension of incubation time, ALP activity in MC3T3-E1 cells in the same experimental group increased, P<0.05. At the same time point, compared with that in the control group, the low concentration of HU-308 (10^-10 mol/L, 10^-9 mol/L) promoted the expression of OPG mRNA, P<0.05. High concentration of HU-308 inhibited the expression of OPG mRNA, and the higher the concentration was, the more significant the inhibition was, P<0.05. The results of phalloidine staining showed that the cells in the 10^-6 mol/L group had the largest spreading area and most explicit structure.Conclusion HU-308 and nHA/PCL electrospun membranes can promote the adhesion, proliferation, and differentiation of MC3T3-E1 cells.

Key words： HU-308 electrospinning nano-hydroxyapatite osteoblast

Received: 07 June 2020

PACS:

R783.1

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	Articles by authors
	QIAO Luhui
	ZHANG Yuntao
	YANG Zhongxue
	MA Ziyu
	HOU Yudong

Cite this article:

QIAO Luhui,ZHANG Yuntao,YANG Zhongxue, et al. Effects of HU-308 and nHA/PCL electrospun membrane on the biological properties of MC3T3-E1[J]. 滨州医学院学报, 2020, 43(6): 438-442.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.06.011 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I6/438

[1] SHIMOMURA K,ROTHRAUFF B B,HART D A,et al.Enhanced repair of meniscal hoop structure injuries using an aligned electrospun nanofibrous scaffold combined with a mesenchymal stem cell-derived tissue engineered construct[J].Biomaterials,2019,192:346-354.
[2] YAO Q,COSME J G,XU T,et al.Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation [J].Biomaterials,2017,115:115-127.
[3] HASSAN M I.Characterization,drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane[J].3 Biotech,2017,7(4): 249.
[4] RAHMANI A,HASHEMI-NAJAFABADI S,ESLAMINEJAD M B,et al.The effect of modified electrospun PCL-nHA-nZnO scaffolds on osteogenesis and angiogenesis[J].J Biomed Mater Res A,2019,107(9):2040-2052.
[5] QIU S,ZHAO F,TANG X,et al.Type-2 cannabinoid receptor regulates proliferation,apoptosis,differentiation,and OPG/RANKL ratio of MC3T3-E1 cells exposed to Titanium particles[J].Mol Cell Biochem,2015,399(1-2):131-141.
[6] SOPHOCLEOUS A,MARINO S,LOGAN J G,et al.Bone cell-autonomous contribution of type 2 cannabinoid receptor to breast cancer-induced osteolysis[J].J Biol Chem,2015,290(36):22049-22060.
[7] APOSTU D,LUCACIU O,MESTER A,et al.Cannabinoids and bone regeneration[J].Drug metabolism reviews,2019,51(1): 65-75.
[8] OSSOLA C A,SURKIN P N,MOHN C E,et al.Anti-inflammatory and osteoprotective effects of cannabinoid-2 receptor agonist hu-308 in a rat model of lipopolysaccharide-induced periodontitis[J].J Periodontol,2016,87(6):725-734.
[9] PALMER L C,NEWCOMB C J,KALTZ S R,et al.Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel[J].Chemical reviews,2008,108(11): 4754-4783.
[10] 余程,刘凯,侯长江,等.3D打印纳米羟基磷灰石/聚己内酯支架生物相容性[J].青岛大学医学院学报,2017,53(1):1-4.
[11] 江新葵,李佳亿,冯青,等.HU-308药物缓释涂层对骨质疏松大鼠种植体周围骨改建相关因子表达的影响[J].中华老年口腔医学杂志,2016,14(2):109-114.
[12] OFEK O,KARSAK M,LECLERC N,et al.Peripheral cannabinoid receptor,CB2,regulates bone mass[J].Proc Natl Acad Sci U S A,2006,103(3):696-701.
[13] 裴方,周冰,郑伟,等.大麻素2型受体激活对钛颗粒诱导成骨细胞凋亡的干预作用[J].中国组织工程研究,2016,20(43): 6431-6437.
[14] 范迎赛,李琴,高宗勤,等.制何首乌水提液对小鼠成骨细胞MC3T3-E1增殖、分化及OPG-RANKL mRNA表达的影响[J].动物医学进展,2015,36(8):67-70.
[15] 李友瑞.富含nHA并缓释NELL-1蛋白的纳米纤维支架构建及其体外生物活性研究[D].山东大学,2018.
[16] 张宇,侯玉东,张云涛,等.HU-308对高浓度糖皮质激素作用下MC3T3-E1成骨细胞生物学性能的影响[J].口腔医学研究,2019,35(1):98-102.
[17] JOHNSON H E,KING S J,ASOKAN S B,et al.F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling[J].J Cell Biol,2015,208: 443-455.