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| Preparation of acellular matrix facial nerve repair material |
| ZHANG Yaqun, ZHANG Naili, ZHAO Dongmei, LIU Hongfu* |
| Department of Anatomy, School of Basic Medicine, Binzhou Medical University, Yantai 264003, Shandong, P.R.China |
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Abstract Objective To prepare the acellular matrix facial nerve repair material and construct a tissue-engineered facial nerve with a natural neural tissue structure which can be used for the repair of peripheral nerve injury.Methods The rabbit facial nerve was taken, and acellular nerve matrix was prepared by chemical extraction, the degree of decellation and demyelination was observed by HE staining.The DNA content of the cells in the scaffold after staining with Quant-iT PicoGreen working solution were measured by fluorescence spectrophotometer. The relative growth rate of cells on the scaffold was determined by MTT to detect the cytotoxicity.Results The scaffold graft was cylindrical, and its elasticity was basically consistent with normal nerves. Tissue observation showed that the structure of cell, the nerve myelin sheath and axon had been completely removed. The extracellular matrix formed a longitudinal arrangement structure and was filled with voids. The residual DNA content in the rabbit acellular facial nerve matrix scaffold was significantly lower than that in the normal rabbit facial nerve. Nerve matrix donors were non-cytotoxic.Conclusion The modified chemical extraction method can effectively remove facial nerve cells, preserve the natural structure intact and have low cytotoxicity. It can be used as a scaffold for tissue engineering facial nerves.
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Received: 23 February 2020
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