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| Effect of IL-17A on the expression of gelatinase in osteoblasts MC3T3-E1 cells and its mechanism |
| DU Xinyuan1, SONG Yan2, XU Lu1, JI Yuhan1, JIANG Tianzi1, SHI Tala1, MI Wei1* |
1 Nutrition and Food Hygiene Section, Binzhou Medical University, Yantai 264003, Shandong, P.R.China;
2 Department of Stomatology, Municipal Hospital of traditional Chinese Medicine and Shandong Provincial Hospital,Jinan 250021, Shandong |
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Abstract Objective To investigate the effect of IL-17A on gelatinase expression in MC3T3-E1 cells and to explore its mechanism.Methods MC3T3-E1 cells were stimulated by IL-17A. The expression of MMP-2 and MMP-9 at mRNA and protein levels were detected by Real-time PCR and ELISA respectively. Immunofluorescence and Western Blot were used to detect the IL-17A-induced activity of NF-κB. Chemical inhibitor of NF-κB was used to detect the involvement of NF-κB in IL-17A-induced MMP-9 expression in MC3T3-E1 cells. Results IL-17A upregulated MMP-9 expression at both mRNA and protein levels (P＜0.01), but had no effect on MMP-2 expression; IL-17A promoted phosphorylation of NF-κB (P＜0.01); inhibition of NF-κB attenuated IL-17A-induced MMP-9 expression(P＜0.05). IL-17A did not affect the TIMP-1 and TIMP-2 expression at mRNA level.Conclusion IL-17A can promote the secretion of MMP-9 by MC3T3-E1 cells by activating NF-κB.
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Received: 10 November 2019
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