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| The significance of DNA quantitative analysis combined with hMSH6 in cervical cancer screening |
| WANG Nana1, ZHANG Qian1*, GAO Shuhong2, WU Shuhua1, WANG Yutang3 |
1 Department of Pathology, Binzhou Medical University Hospital, Binzhou 256603, Shandong, P.R.China;
2 Department of Gynecology, Binzhou Medical University Hospital;
3 School of Clinical Medicine , Binzhou Medical University |
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Abstract Objective To investigate the correlation and clinical significance of DNA ploidy analysis with hMSH6 and HPV in cervical cancer. Methods High risk HPV-DNA detection and immunohistochemical hMSH6 staining were used to analyze the expression of hMSH6, HPV infection and correlation in cervical tissues with different lesions. Results The specificity of cervical lesions was 25.86% and 77.59%, respectively, based on the presence of DNA aneuploidy and moderate amount of DNA aneuploidy. It suggests that the presence of more than moderate amount of DNA aneuploidy has a higher diagnostic value for cervical lesions screening. The positive rates of hMSH6 were 75% and 85.71% in the multidose and medium dose alloploid groups, which had statistically significant differences from the control group (P＜0.05). The infection rate of HPV was 92.86% and 76.19% in the multi-dose and medium-dose DNA aneuploidy groups,which showing statistically significant differences from the control group (P＜0.05). The MSH6 positive rate of the multiploidy abnormal HPV infection group was 90.38%, which was different from the MSH6 positive rate of the multiploidy abnormal HPV infection group 25% (P＜0.05) Conclusion HPV combined with hMSH6 test is the detection of etiology, with high sensitivity. The high expression of hMSH6 protein caused by HPV infection is expected to provide a reference for the prevention and treatment of cervical cancer.
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Received: 28 June 2019
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