Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells

ZHOU Chao; SUN Congcong; ZHANG Youzhong; LIU Zhiqiang; ZHANG Yingzi

doi:10.19739/j.cnki.issn1001-9510.2020.02.001

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Journal of Binzhou Medical University ›› 2020, Vol. 43 ›› Issue (2) : 81-86. DOI: 10.19739/j.cnki.issn1001-9510.2020.02.001

Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells

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Abstract

Objective To investigate the expression of miR-212 in cervical cancer and its effect on proliferation and invasion of cervical cancer cells and its mechanism. Methods RT-PCR and Western Blot were used To detect the expression levels of miR-212 and TCF7L2 in cervical cancer cells. BrdU cell proliferation assay and Transwell invasion assay were used to detect cell proliferation and invasion. Target Scan was used to predict the protein-coding gene target of miR-212, and double luciferase reporting system was used to validate it. Results The expression of mir-212 in cervical cancer tissues was down-regulated, compared with that in adjacent tissues (P<0.01). Compared with End1/E6E7 cells, the expression level of mir-212 in cervical cancer cell lines was significantly reduced (P<0.01). Overexpression of miR-212 can inhibit cell proliferation and invasion, while low expression of miR-212 can promote the proliferation and invasion of cervical cancer cell lines (P<0.01). Overexpression of miR-212 can significantly inhibit the expression of TCF7L2 protein, while low expression of miR-212 can promote the expression of TCF7L2 protein. The target gene TCF7L2 is the direct target of miR-212. Conclusion The expression of miR-212 in cervical cancer tissue was decreased, and the invasion and metastasis of cervical cancer were inhibited by targeting regulation of TCF7L2 gene.

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miR-212 / TCF7L2 / cervical cancer / proliferation / invasion

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ZHOU Chao, SUN Congcong, ZHANG Youzhong, et al. Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells[J]. Journal of Binzhou Medical University, 2020, 43(2): 81-86 https://doi.org/10.19739/j.cnki.issn1001-9510.2020.02.001

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