miR-212 在宫颈癌中的表达及对癌细胞增殖和侵袭的影响与机制

doi:10.19739/j.cnki.issn1001-9510.2020.02.001

Abstract
Figure/Table
References
Related Citation (7)

Download: PDF (1109 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the expression of miR-212 in cervical cancer and its effect on proliferation and invasion of cervical cancer cells and its mechanism. Methods RT-PCR and Western Blot were used To detect the expression levels of miR-212 and TCF7L2 in cervical cancer cells. BrdU cell proliferation assay and Transwell invasion assay were used to detect cell proliferation and invasion. Target Scan was used to predict the protein-coding gene target of miR-212, and double luciferase reporting system was used to validate it. Results The expression of mir-212 in cervical cancer tissues was down-regulated, compared with that in adjacent tissues (P<0.01). Compared with End1/E6E7 cells, the expression level of mir-212 in cervical cancer cell lines was significantly reduced (P<0.01). Overexpression of miR-212 can inhibit cell proliferation and invasion, while low expression of miR-212 can promote the proliferation and invasion of cervical cancer cell lines (P<0.01). Overexpression of miR-212 can significantly inhibit the expression of TCF7L2 protein, while low expression of miR-212 can promote the expression of TCF7L2 protein. The target gene TCF7L2 is the direct target of miR-212. Conclusion The expression of miR-212 in cervical cancer tissue was decreased, and the invasion and metastasis of cervical cancer were inhibited by targeting regulation of TCF7L2 gene.

Key words： miR-212 TCF7L2 cervical cancer proliferation invasion

Received: 28 June 2019

PACS:

R737.33

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHOU Chao
	SUN Congcong
	ZHANG Youzhong
	LIU Zhiqiang
	ZHANG Yingzi

Cite this article:

ZHOU Chao,SUN Congcong,ZHANG Youzhong, et al. Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells[J]. 滨州医学院学报, 2020, 43(2): 81-86.

URL:

http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.02.001 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I2/81

[1] FERLAY J, SOERJOMATARAM I, DIKSHIT R, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012[J]. Int J Cancer, 2015, 136(5):359-386.
[2] JELASTOPULU E, FAFLIORA E, PLOTA A, et al. Knowledge, behaviours and attitudes regarding HPV infection and its prevention in female students in West Greece[J]. Eur RevMed Pharmacol Sci, 2016, 20(12): 2622-2629.
[3] IADEVAIA V, GERBER A P. Combinatorial control of mRNA fates by RNA-binding proteins and noncoding RNAs[J]. Biomolecules, 2015, 5(4): 2207-2222.
[4] KONG Y W, FERLAND-MCCOLLOUGH D, JACKSON T J, et al. microRNAs in cancer management[J]. Lancet Oncol, 2012, 13(6): 249-258.
[5] KIM V N. MicroRNA biogenesis: coordinated cropping and dicing[J]. Nat Rev Mol Cell Biol, 2005, 6(5):376-385.
[6] BARTEL D P. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2): 281-297.
[7] ZHAO B, DONG A S. MiR-874 inhibits cell growth and induces apoptosis by targeting STAT3 in human colorectal cancer cells[J]. Eur Rev Med Pharmacol Sci, 2016, 20(2): 269-277.
[8] ZHANG H, YU C, CHEN M, et al. miR-522 contributes to cell proliferation of hepatocellular carcinoma by targeting DKK1 and SFRP2[J]. Tumour Biol, 2016, 37(8): 11321-11329.
[9] 章燕,祝亚平,鲍伟.下调microRNA-107表达对人子宫内膜样腺癌Ishikawa细胞增殖迁移和侵袭的作用 [J]. 现代妇产科进展,2018,27(5):330-333.
[10] LI B, YANG X X, WANG D, et al. MicroRNA-138 inhibits proliferation of cervical cancer cells by targeting c-Met[J]. Eur Rev Med Pharmacol Sci, 2016, 20(6):1109-1114.
[11] YANG Y, JIA D, KIM H, et al. Dysregulation of miR-212 promotes castration resistance through hnRNPH1-mediated regulation of AR and AR-V7: implications for racial disparity of prostate cancer[J]. Clin Cancer Res, 2016, 22(7):1744-1756.
[12] ZHAO J L, ZHANG L, GUO X, et al. miR-212/132 downregulates SMAD2 expression to suppress the G1/S phase transition of the cell cycle and the epithelial to mesenchymal transition in cervical cancer cells[J]. IUBMB Life, 2015, 67(5): 380-394.
[13] JIANG Z, SONG Q, ZENG R, et al. MicroRNA-218 inhibits EMT, migration and invasion by targeting SFMBT1 and DCUN1D1 in cervical cancer[J]. Oncotarget, 2016, 7(29):45622-45636.
[14] DOU C, WANG Y, LI C, et al. MicroRNA-212 suppresses tumor growth of human hepatocellular carcinomaby targeting FOXA1[J]. Oncotarget, 2015, 6(15): 13216-13228.
[15] LI D, LI Z, XIONG J, et al. MicroRNA-212 functions as an epigenetic-silenced tumor suppressor involving in tumor metastasis and invasion of gastric cancer through down-regulating PXN expression[J]. Am J Cancer Res, 2015, 5(10): 2980-2997.
[16] 裴祎, 张晓晶, 郑珂, 等. miR-212抑制骨肉瘤细胞恶性生物学行为的实验研究[J].中国医科大学学报,2018,47(10):924-928.
[17] 李耀辉,刘春来. miRNA-212在膀胱癌中的表达及其上调对癌细胞增殖、迁移、侵袭的影响与机制 [J]. 山东医药,2018,58(46):7-10.
[18] QI B, LIU S G, QIN X G, et al. Overregulation of microRNA-212 in the poor prognosis of esophageal cancer patients[J]. Genet Mol Res, 2014, 13(3): 7800-7807.
[19] MENG X, WU J, PAN C, et al. Genetic and epigenetic down-regulation of microRNA-212 promotes colorectal tumor metastasis via dysregulation of MnSOD[J]. Gastroenterology, 2013, 145(2):426-436.
[20] MA C, NONG K, WU B, et al. miR-212 promotes pancreatic cancer cell growth and invasion by targeting the hedgehog signaling pathway receptor patched-1[J]. J Exp Clin Cancer Res, 2014, 33: 54.
[21] BOJ S F, VAN E S J H, HUCH M, et al. Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand[J]. Cell, 2012, 151(7): 1595-1607.
[22] ZHENG Z, LIU J, YANG Z, et al. MicroRNA-452 promotes stem-like cells of hepatocellular carcinoma by inhibiting Sox7 involving Wnt/β-catenin signaling pathway[J]. Oncotarget, 2016, 7(19): 28000-28012.
[23] GU W, LI X, WANG J. miR-139 regulates the proliferation and invasion of hepatocellular carcinoma through the WNT/TCF-4 pathway[J]. Oncol Rep, 2014, 31(1): 397-404.
[24] WANG X, XIA Y. microRNA-328 inhibits cervical cancer cell proliferation and tumorigenesis by targeting TCF7L2[J]. Biochem Biophys Res Commun, 2016, 475(2): 169-175.
[25] 付翠群,丁西平,沈国栋,等.转录因子7类似物2在胃癌中的表达及其与铂类药物耐药性的关系[J].中国临床保健杂志,2018,21(1):92-96.
[26] 王昕楠.LncRNA SPRY4-IT1在乳腺癌干细胞中作为ceRNA调控TCF7L2表达的作用和分子机制[D].中国医科大学,2018.