参附注射液对血管性痴呆大鼠空间学习记忆功能和海马组织病理学改变的作用

doi:10.19739/j.cnki.issn1001-9510.2019.06.001

Abstract
Figure/Table
References
Related Citation (1)

Download: PDF (1808 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To observe the effect of Shenfu injection (SF) on learning and memory ability and pathological changes of hippocampus in vascular dementia (VD) rats. Methods The VD models, established by repeatedly clipping the common carotid arteries (CCA) of the rat in combination with an intraperitoneal injection of sodium nitroprusside solution in anesthetized SD rats, were randomly divided into two groups: model group and SF treated group, and another condition-matched rats were selected as the sham-operated group in which the bilateral CCA were separated but with neither CCA occlusion nor intraperitoneal injection of sodium nitroprusside solution. Above 3 groups were further divided into three time point subgroups respectively: 7d, 15d and 1m subgroups after model-established operation. The SF treated group were intraperitoneally treated with SF (10mL/kg/d), while the model and sham-operated groups were given the same volume of normal saline as SF treated group. At different time point, spatial learning and memory abilities of rats were assessed by Morris water maze task and the microcosmic pathological changes in hippocampal CA1 area were comparatively observed in HE straining, Nissl staining slices through microscopy and transmission electron microscopy. Results The escape latency (EL) of VD rats was significantly higher than sham operation group (P<0.01) While reduced (P<0.05) in SF treated rats. Pathology testing showed that pyramidal cells in CA1 area of hippocampus reduced or even dropped out in VD rats. At the same time, cells were swelling with nucleus pyknosis. Moreover, the number of Nissl bodies reduced or even disappeared, accompanied by glial cells hyperplasia. With treatment of SF, the damages mitigated. Transmission electron microscopy showed damages of integrity and structure in hippocampus of VD rats (1m), accompanied by a decrease in the number of synaptic vesicles while SF relieved the damages. Conclusion SF can ameliorate dementia symptoms and enhance learning and memory in VD rats to some extent.

Key words： Senfu injection vascular dementia pathology changes learning and memory ability

Received: 14 October 2019

PACS:

R338.6

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Naixuan
	LIU Hongxia
	PAN Hong
	SHI Zhen
	YU Lamei

Cite this article:

LI Naixuan,LIU Hongxia,PAN Hong, et al. Effect of shenfu injection on spatial learning and memory ability and hippocampal pathological changes in vascular dementia rats[J]. 滨州医学院学报, 2019, 42(6): 401-406.

URL:

http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2019.06.001 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2019/V42/I6/401

[1] O'Brien J T, Thomas A. Vascular dementia [J]. Lancet, 2015, 386(10004):1698-1706.
[2] 杨萍, 李华, 章永南, 等. 参附注射液治疗舒张性心力衰竭的临床疗效[J]. 中国老年学杂志, 2016, 36(9):2103-2105.
[3] 王露, 罗科, 卿国忠, 等. 参附注射液对急性创伤性脑损伤大鼠脑保护作用的研究[J]. 中国急救医学,2017, 37(11):1041-1045.
[4] 龙小菊, 梁应平, 胡衍辉, 等. 参附注射液预处理联合控制性低中心静脉压对肝脏缺血-再灌注损伤的影响[J].临床麻醉学杂志, 2014, (6);536-539.
[5] 柏本健, 田永仓, 康小琴, 等. 参附注射液灌胃对 MIRI 模型大鼠 Ca²⁺、KATP 通道的影响[J]. 临床医学研究与实践, 2018, 3(26):5-6.
[6] 张国欣, 张凌云, 谭萍, 等. 参附注射液对大鼠胆道缺血再灌注损伤的保护作用[J]. 中国民族民间医药, 2019, 28 (1):15-19.
[7] 邢雪松, 吕威力, 吴溪婷. 不同干预方法对血管性痴呆大鼠神经再生及脑组织N-myc蛋白表达的影响[J] . 中风与神经疾病杂志, 2015, 32(7):616-618.
[8] 黄艳红, 辛晓燕, 陈亚琼, 等. 染料木素和17β-雌二醇对卵巢切除大鼠学习与记忆的影响[J]. 第四军医大学学报, 2004, 25(1):46-49.
[9] Otori T, Katsumata T, Muramatsu H, et al. Long-term measurement of cerebral blood flow and metabolism in a rat chronic hypoperfusion model[J]. Clin Exp Pharmacol Physiol, 2003, 30(4):266-272.
[10] Choy M, Ganesan V, Thomas D L, et al. The chronic vascular and haemodynamic response after permanent bilateral common carotid occlusion in newborn and adult rat[J]. J Cereb Blood Flow Metab, 2006, 26(8):1066-1075.
[11] Morris R. Developments of a water-maze procedure for studying spatial learning in the rat[J]. J Neurosci Methods, 1984, 11(1):47-60.
[12] Fischer W, Chen K S, Gage F H, et a1. Progressive decline in spatial learning and integrity of forebrain cholinergic neurons in rats during aging[J]. Neurobiol Aging, 1992, 13(1):9-23.
[13] 李长喜, 吴莉峰, 曾贵荣, 等. 参附注射液对大鼠脑缺血再灌注损伤的保护作用研究[J]. 中国医师杂志,2018, 20(10):1487-1490.
[14] 江承平, 吴碧华, 王柏强, 等. 参附注射液对脑缺血再灌注损伤大鼠Nrf2信号通路的影响[J]. 中国免疫学杂志, 2015(9):1191-1194.
[15] 于腊梅, 李乃选, 王桂华, 等. 参附注射液对大鼠脑缺血再灌注后海马CA1区Caspase-3表达的影响[J]. 滨州医学院学报, 2011, 34(3):179-181.
[16] 于腊梅, 杨丽娟, 蒋淑君. 参附注射液对大鼠脑缺血再灌注后Bcl-2和Bax蛋白表达的影响[J]. 滨州医学院学报, 2010, 33(2):93-94.