N-精氨酰壳寡糖聚合物纳米粒的构建及其作为药物载体的可行性研究

doi:10.19739/j.cnki.issn1001-9510.2019.05.014

Abstract
Figure/Table
References
Related Citation (0)

Download: PDF (1689 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To prepare N-arginyl-chitooligosaccharide nanoparticles (ACOS NPs) and study thefeasibility of ACOS NPs as drug carriers. Methods N-arginyl-chitooligosaccharide(ACOS) was synthesized and analyzed by FT-IR and 1HNMR. ACOS NPs were prepared by ionic gelation method and characterized by TEM and ZetasizerNano-ZS. The in vitro cytotoxicity profiles of ACOS NPs againstHeLa cells were performed by MTT assay. The cellular uptake profiles of ACOS NPs byHeLa cells were determined by flow cytometry (FCM) and viewed by confocallaser scanning microscopy (CLSM). Results The FT-IR and 1H NMR Results confirmed the formation of ACOS. ACOS NPs were spherical in shape with a mean particle size of 146.3 nm and zeta potential of 9.76 mV and well-dispersed. ACOS NPs possessed no cytotoxicity to HeLa cells in vitro, indicating their good biocompatibility. ACOS NPs could be internalized rapidly and continuously by HeLa cells with a high uptake rate, and the cellular uptake was time- and concentration-dependent. Conclusion ACOS NPs with good biocompatibility can significantly improve the intracellular delivery efficacy of drugs,therefore are promising to be used as drugcarriers with low cytotoxicity and high efficacy.

Key words： arginine chitooligosaccharide nanoparticle drug carriers

Received: 18 March 2019

PACS:

R917

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LUO Tingting
	ZHANG Jing

Cite this article:

LUO Tingting,ZHANG Jing. Preparation and Feasibility Study of Polymeric Nanoparticles Based on N-arginyl-chitooligosaccharide as Drug Carriers[J]. 滨州医学院学报, 2019, 42(5): 367-372.

URL:

http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2019.05.014 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2019/V42/I5/367

[1] M G A, S A T, Ayyavu M, et al. Synthesis and characterization of cystamine conjugated chitosan-SS-mPEG based 5-Fluorouracil loaded polymeric nanoparticles for redox responsive drug release[J]. Eur J Pharm Sci, 2018,116: 37-47.
[2] Yu Y, Huo M, Fu Y, et al. N-Deoxycholic acid-N,O-hydroxyethylchitosan with a sulfhydryl modification to enhance the oral absorptive efficiency of paclitaxel [J]. Mol Pharm, 2017, 14(12):4539-4550.
[3] Chen S, Luo C, Wen W, et al. Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer [J]. Mater Sci Eng C Mater Biol Appl, 2018, 93: 319-331.
[4] Kim E K, Je J Y, Lee S J, et al. Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells [J]. Bioorg Med Chem Lett, 2012, 22(19):6136-6138.
[5] Sánchez Á, Mengíbar M, Fernández M, et al. Influence of preparation methods of chitooligosaccharides on their physicochemical properties and their anti-inflammatory effects in mice and in RAW264.7 macrophages [J]. Mar Drugs, 2018, 16(11): 430.
[6] Amit C, Muralikumar S, Janaki S, et al. Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system [J]. Int J Nanomedicine, 2019, 14: 605-622.
[7] Guo F, Ouyang T, Peng T, et al. Enhanced oral absorption of insulin using colon-specific nanoparticles co-modified with amphiphilic chitosan derivatives and cell-penetrating peptides [J]. Biomater Sci, 2019, 7(4):1493-1506.
[8] Gagat M, Zielińska W, Grzanka A. Cell-penetrating peptides and their utility in genome function modifications (Review)[J]. Int J Mol Med, 2017, 40(6):1615-1623.
[9] Mohammadi S, Zakeri-Milani P, Golkar N, et al. Synthesis and cellular characterization of various nano-assemblies of cell penetrating peptide-epirubicin-polyglutamate conjugates for the enhancement of antitumor activity [J]. Artif Cells Nanomed Biotechnol, 2018, 46(8):1572-1585.
[10] Bode S A, Timmermans S B P E, Eising S, et al. Click to enter: activation of oligo-arginine cell-penetrating peptides by bioorthogonaltetrazineligations[J].Chem Sci, 2018, 10(3):701-705.
[11] 刘萍, 何文, 汪静, 等. 寡聚精氨酸壳聚糖对替硝唑透皮吸收促进作用的体内外研究[J]. 中国药师, 2015(5): 707-711.
[12] 万丽卿, 胡富强, 袁弘. A549细胞对壳寡糖及其纳米粒的摄取作用[J]. 药学学报, 2004, 39(3): 227-231.
[13] 徐晓龙. 精氨酸—壳聚糖/DNA纳米粒控释PELA微球的制备及其成骨诱导效能的实验研究[D]. 广州:南方医科大学, 2016.
[14] 何文, 刘贝, 郭咸希.不同取代度的寡聚精氨酸壳聚糖的合成与鉴定[J].安徽医药, 2014, 18(5):826-829.
[15] Jeon Y O, Lee J S, Lee H G. Improving solubility, stability, and cellular uptake of resveratrol by nanoencapsulation with chitosan and γ-poly (glutamic acid) [J]. Colloids Surf B Biointerfaces, 2016, 147: 224-233.