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| Effects of chloroquine on neurotransmitter expression in rat brain with epilepsy induced by pentylenetetrazole |
| YU Ning1, ZHAO Yuhong1, JING Lili2, WU Shuhua3, CAO Zhang3 |
1 Department of Pathology, Binzhou Medical University Hospital, Binzhou 256603, P.R.China;
2 Department of Pathology, Yantai Affiliated Hospital of Binzhou Medical University;
3 Department of Pathology,Binzhou Medical University |
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Abstract Objective To observe the effects of chloroquine on adenosine kinase (ADK) and Glutamate (Glu)in cerebral cortex and hippocampal of rats with epilepsy induced by pentylenetetrazole, to explore the role of chloroquine in epileptogenesis. Methods Thirty male adult Sprague-Dawley (SD) rats were randomly divided into control group (n=10), pentylenetetrazole (PTZ) group (n=10) and chloroquine intervening group (n=10). The behavior and electroencephalogram of rats were observed and recorded. The expression of ADK and Glu in cerebral cortex and hippocampus was examined with immunohistochemistry. Results The control group showed no seizure activity. Severe seizure activity was observed in PTZ group (Ⅳ-Ⅴdegree), while slight seizure activity(Ⅰ-Ⅲdegree) appeared in the chloroquine intervening group. Electroencephalogram studies showed high amplitudes with fast frequencies in the PTZ group, low amplitudes and slow frequencies in the chloroquine intervening group. The expressions of ADK and Glu in the PTZ group were stronger than that of the control group (P<0.01), and significant differences were observed between the chloroquine intervening group and the PTZ group (P<0.05). Conclusion Chloroquine could alleviate the seizure activities induced by PTZ in rats, inhibit the expression level of ADK and Glu.
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Received: 26 February 2019
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