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| VNTR analysis of molecular typing of Pseudomonas aeruginosa in nosocomial infection |
| MENG Wei1, LIU Yongyun2, JI Bing2, LI Jinling2, AN Xinye2 |
1 Department of Clinical Medicine Laboratory,Binzhou Medical University Hospital,Binzhou 256603,P.R.China;
2 Clinical Laboratory,Binzhou Medical University Hospital |
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Abstract Objective To investigate the effect of multi-loci VNTR analysis on molecular typing of multi-drug-resistance pseudomonas aeruginosa.Methods Seventy-eight strains of multi-drug-resistance pseudomonas aeruginosa were isolated from 13 departments of our hospital from May 2015 to May 2017,and their DNA was extracted.Eight sites of pseudomonas aeruginosa were randomly selected from the genome of pseudomonas aeruginosa,and the DNA was amplified by VNTR amplification.The products were extracted by capillary electrophoresis.The relative molecular mass of the amplified product and the number of VNTR repeats at different sites of the strain were calculated.Multidrug-resistant pseudomonas aeruginosa was subjected to cluster analysis and homology analysis based on the relative molecular mass of the amplified product and the number of VNTR repeats at different sites.Results The repeat base sequence size of 8 VNTR sites was 7-128 bp,the polymorphism index was 0.47-0.89,and the distribution was relatively uniform.VNTR analysis was applied to divide 78 multi-drug-resistance pseudomonas aeruginosa into 5 groups.In 6 patients,the repeat number of different isolates was the same at 8 sites.Conclusion VNTR analysis method has high application value in the genotyping study and the control of epidemic tracing of pseudomonas aeruginosa. It can provide a basis and technical method for the control of nosocomial infection, which requires high attention.
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Received: 18 August 2018
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