反复自然流产患者主动免疫治疗后封闭抗体和调节性T细胞水平变化

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Abstract Objective To investigate the pregnancy outcome by active immunotherapy for recurrent spontaneous abortions(RSA) and the changes of blocking antibody (BA) and the levels of CD₄⁺CD₂₅⁺T cells and CD₄⁺CD₂₅^highTreg cells in peripheral blood after immunotherapy.Methods Detection of BA by micro lymphocytic toxicity test (MLCT),300 negative patients randomly selected were treated by active immunotherapy of lymphocytes, and the pregnancy outcomes were followed. Blocking efficiency and the levels of CD₄⁺CD₂₅⁺T cells and CD₄⁺CD₂₅^highTreg cells in peripheral blood were detected by flow cytometry in 100 cases randomly selected.Results Of the 300 patients receiving active immunotherapy,265 were pregnant,and 35 were still in the immunotherapy.In the patients with pregnancy,10 cases had an early abortion,165 had successful delivery and 90 were still pregnant, and the success rate of the active immunotherapy was 96.2%. The level of BA was significantly higher after active immunotherapy (37.73±2.42%) than that before immunotherapy (7.25±0.39%) in 100 patients.The blocking efficiencies of CD₃,CD₄ and CD₂₅ were higher than that before immunotherapy,and CD₃ and CD₂₅ increased very significantly (P＜0.01).The ratios of CD₄⁺CD₂₅⁺T cells and CD₄+CD₂₅^highTreg cells were significantly higher than that before active immunotherapy,and the difference was statistically significant(P＜0.01,P＜0.05).Conclusion The occurrence of RSA may be related to the decreases of blocking antibody,CD₄⁺CD₂₅⁺T cells and CD₄⁺CD₂₅^highTreg cells in the peripheral blood of the patients.Lymphocyte active immunotherapy can significantly improve the levels of blocking antibody and the ratios of CD₄⁺CD₂₅⁺T cells and CD₄⁺CD₂₅^highTreg cells in peripheral blood. It is a safe and effective way to treat RSA.

Key words： active immunotherapy recurrent spontaneous abortion blocking antibody CD₄⁺CD₂₅⁺T cell CD₄⁺CD₂₅^highTreg

Received: 13 April 2018

PACS:

R714.21

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	Articles by authors
	ZHENG Jing
	ZHUO Yue
	LIU Yanni

Cite this article:

ZHENG Jing,ZHUO Yue,LIU Yanni. Changes in the levels of blocking antibody and regulatory T cells after active immunotherapy inpatients with recurrent spontaneous abortion[J]. 滨州医学院学报, 2018, 41(4): 255-258.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/ OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2018/V41/I4/255

[1] 王欣,李培旭.封闭抗体阴性的反复自然流产患者采用配偶淋巴细胞主动免疫治疗效果观察[J].生殖医学杂志,2016,25(11):998-1001.
[2] Rocklin R E,Kitzmiller J L,Carpenter C B,et al.Maternal-fetal relation.Absence of an immunologic blocking factor from the serum of women with chronic abortions[J].N Engl J Med,1976,295(22):1209-1213.
[3] Yang Y,Huang C T,Huang X,et al.Persistent Toll-like receptor signals are required for reversal of regulatory T cell-mediated CD₈ tolerance[J].Nat Immunol,2004,5(5):508-515.
[4] 王燕,林娜,陈雪美,等.反复自然流产患者淋巴细胞主动免疫的治疗效果及其与HLA相关性研究[J].海南医学,2018,29(1):59-61.
[5] Taylor C,Faulk W P.Prevention of recurrent abortion with leucocyte transfusions[J].Lancet,1981,2(8237):68-70.
[6] 焦艳,王蒙,李欣.150例反复性自然流产患者封闭抗体的检测与治疗分析[J].国际检验医学杂志,2018,39(6):744-746.
[7] 陈雪美,黄海龙,陈小兰,等.反复流产患者73例淋巴细胞主动免疫治疗的护理[J].福建医药杂志,2017,39(5):161-162.
[8] 刘艳秋,包娟,周萍.原因不明反复自然流产患者的主动免疫治疗[J].江西医药,2009,44 (2):98-100.
[9] 石瑛,泰淑红,荣守华,等.利用流式细胞术检测T淋巴细胞表面分子CD₃、CD₄和CD₈封闭效率在反复自然流产中的应用 [J].国际检验医学杂志,2014,35(6):682-683.
[10] 许园园,刘雨生.原因不明性复发性流产患者主动免疫治疗效果及调节性T细胞水平变化观察[J].安徽医学,2009,30(10):1135-1137.
[11] 刘雨生,赵淑云,季静娟,等.原因不明复发性流产患者调节性T淋巴细胞比例及功能的测定[J].中华妇产科杂志,2007,42 (3):176-179.
[12] Tilburgs T,Roelen D L,van der Mast B J ,et al.Evidence for a selective migration of fetus-specific CD₄⁺CD₂₅bright regulatory T cells from the peripheral blood to the decidua in human pregnancy[J].J Immunol,2008,180(8):5737-5745.
[13] Somerset D A,Zheng Y,Kilby M D,et al.Normal human pregnancy is associated with an elevation in the immune suppressive CD₂₅⁺ CD₄⁺ regulatory T-cell subset[J].Immunology.2004,112(1):38-43.