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| Effect of transient potential receptor channel protein 6 (TRPC6) on proliferation of SGC-7901 cells |
| GE Pengbo, ZHANG Mingkai, WANG Kai, LI Yanbin, LIU Shuaichen, WANG Jianping, LI Yuming |
| Department of Gastrointestinal Surgery,Binzhou Medical University Hospital,Binzhou 256603,P.R.China |
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Abstract Objective To investigate the expression of TRPC6 in SGC-7901 cells and to elucidate the relationship between TRPC6 protein and proliferation of SGC-7901 cells.Methods SGC-7901 cells were cultured in vitro,and SGC-7901 cells were divided into control group and experimental group with or without TGF-β1 treatment. The experimental groups were divided into TGF-β1 and TGF-β1+SKF-96365 group according to whether incubated with SKF96365 or not.Immunofluorescence staining was used to observe TRPC6 protein expression in SGC-7901 cells in the control and TGF-β1 groups.Western blot was used to detect the protein changes of TRPC6 and proliferation indexes in control group,TGF-β1 group and TGF-β1+SKF96365group.Results Compared with the control group,the expression of TRPC6 protein in TGF-β1 group was significantly increased(P＜0.05).SKF96365 inhibited TRPC6 protein expression,significantly suppressed the proliferation of SGC-7901 cells(P＜0.01).Conclusion Blockade of TRPC6 protein expression could reduce the proliferation ability of SGC-7901 cells.
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Received: 10 March 2018
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