支气管肺泡灌洗液MGMT基因甲基化检测在非小细胞肺癌诊断中的价值

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Abstract Objective To discuss the diagnostic value of the promoter hypermethylation of MGMT gene of Bronchoalveolar lavage fluid (BALF) on non-small cell lung cancer.Methods 98 patients underwent chest surgery were selected as study subjects;63 patients,pathologically diagnosed as non-small cell lung cancer, were enrolled in this study.35 patients with benign lesions were selected as the control group.Serum samples,BALF specimens and biopsy specimens were taken from different groups;MGMT gene promoter methylation level for the specimens was detected.Results The positive rates of promoter methylation of MGMT gene in lung cancer tissues,BALF specimens and the serum samples was 58.7%,52.4% and 27%, respectively.The positive rates of promoter methylation of MGMT gene in lung cancer tissues and the values of BALF specimens coincided with each other.While the consistency of promoter methylation of MGMT gene with lung cancer tissues and that of serum specimens is poor.The sensitivity of benign or malignant tumors detection was lower than that of BALF specimens. In the group of 35 patients with benign lesions,the methylation of the MGMT gen was not detected in the biopsy specimens,the BALF specimens and the serum samples; the incidence of methylation was 0.Conclusion Methylation detection of MGMT gene in BALF had certain clinical application value in the diagnosis of non-small cell lung cancer.

Key words： Bronchoalveolar lavage fluid non-small cell lung cancer MGMT gene methylation

Received: 10 December 2017

PACS:

R734.2

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	Articles by authors
	YUAN Qun
	ZHANG Jianyin
	CHEN Qian
	MA Wei
	ZHANG Miao
	LI Youjin
	ZHUAN Bing

Cite this article:

YUAN Qun,ZHANG Jianyin,CHEN Qian, et al. Values of promoter hypermethylation of MGMT gene in Bronchoalveolar lavage fluid on the diagnosis of non-small cell lung cancer[J]. 滨州医学院学报, 2018, 41(2): 105-108.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/ OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2018/V41/I2/105

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