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| Change of TNF-α in the spinal cord of SOD1-G93A transgenic mice |
| HAN Xiaofeng, QI Yuying, WANG Yuan, MA Wenbin, WANG Xuezhen |
| Department of Neurology,Binzhou Medical University Hospital,Binzhou 256603,P.R.China |
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Abstract Objective To observe the change of TNF-α in the lumbar and thoracic spinal cord of SOD1-G93A transgenic mice at different stages and discuss the relationship between and progression of amyotrophic lateral sclerosis (ALS).Methods The level of TNF-α and TNF-α mRNA was analyzed by ELISA and RT-PCR at presymptomatic,symptomatic and end stages of SODl-G93A transgenic mice.Results Increasing TNF-α and TNF-α mRNA appeared at presymptomatic stage in the lumbar and thoracic spinal cord of SODl-G93A transgenic mice,and became obvious at symptomatic stage and reached the maximum at end stage.Conclusion TNF-α proliferates markedly with the progression symptom of SODl-G93A transgenic mice.TNF-α mediated neuroinfalammation, play an important role in the pathophysiology of ALS.
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Received: 07 June 2017
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