|
|
|
| Peanut Skin Procyanidins reduce the expression of survivin and induce the apoptosis of pancreaticcancer cells through Wnt/ beta-catenin signaling pathway |
| MI Wei1, LIU Jinhui1, GUO Mengzhu1, LIU Chengjuan1, TIAN Cheng1, LI Changzhen2, TANG Jinshuo2 |
1 Nutrition and Food Hygiene Section,Public Health and Management College,Binzhou Medical University,Yantai 264003,P.R.China;
2 Clinical Medicine College, Binzhou Medical University |
|
|
|
|
Abstract Objective The present work was undertaken to explore the molecular mechanism for the apoptosis of pancreatic cancer cells induced by PSP through down regulating the expression of survivin via the Wnt/β-catenin signal pathway.Methods Pancreatic cancer cells were nurtured routinely at PSP concentrations of 100,200,300,400,500 and 600μg/mL.The cell counting kit-8(CCK-8) assay was used to detect and calculate the inhibitory rate of pancreatic cancer cells after 72 hours of culturing.The IC50 of 450μg/mL was chosen as the experimental group. Western blotting was adopted to detect the degree of GSK-3β and β-catenin phosphorylation and the protein expression levels of survivin, caspase-3 and caspase-7.RT-PCR was used to evaluate the expression level of survivin mRNA.Annexin V-FITC/PI was used to test inhibitory rate of pancreatic cancer cells after 24, 48 and 72 hours.Results The degree of GSK-3β and β-catenin phosphorylation reached the highest (P＜0.01) after 12h in the experiment group.The expression of survivin mRNA in the experiment group was significantly lower (P＜0.01) compared to that in the control group.Under the action of PSP,the expression of survivin protein was the lowest (P＜0.01) after 72 hours while the expression of caspase-3 and caspase-7 protein were the highest(P＜0.01)after 72 hours.The apoptosis rate of pancreatic cancer cells increased as the culture period was prolonged,which reached 51.59% (P<0.01) after 72 hours of culture.Conclusion PSP can exert a pro-apoptotic effect on pancreatic cancer cells by reducing the expression level of survivin through the Wnt/β-catenin signal pathway.
|
|
Received: 14 April 2017
|
|
|
|
|
|
[1] Schober M,Jesenofsky R,Faissner R,et al.Desmoplasia and Chemoresistance in Pancreatic Cancer[J].Cancers,2014,6(4): 2137-2154.
[2] Yan L,Wang Y,Wang Z Z,et al.Cell motility and spreading promoted by CEACAM6 through cyclin D1/CDK4 in human pancreatic carcinoma[J].Oncology Reports,2016,35(1):418-426.
[3] 郭世奎,龚昆梅,肖乐,等.胰腺癌相关信号转导通路研究新进展[J].中国肿瘤临床与康复,2010,17(6):563-565.
[4] Lucero O M,Dawson D W,Moon R T,et al.A re-evaluation of the “oncogenic” nature of Wnt/beta-catenin signaling in melanoma and other cancers[J].Current Oncology Reports,2010,12(5):314-318.
[5] Samoski P J,Johnson J V,Reed K A,et al.Separation and characterisation of proanthocyanidins in Virginia type peanut skins by LC-MS n[J].Food Chemistry,2012,131(3):927-939.
[6] 张慧君,辛德慧,孙思睿,等. 响应面法优化四粒红花生衣中原花青素的提取工艺[J].食品工业,2015,36(9):79-82.
[7] 陈洋,王冰,玉佳男,等.RP-HPLC-ESI-MS/MS分离鉴定花生红衣原花青素A型和B型二聚体[J].食品科学,2013,34(23):142-146.
[8] 赵艳敏,王志平,刘岱琳.葡萄籽和花生红衣中原花青素的差异性研究[J].食品研究与开发,2014,35(3):65-68,84.
[9] 宓伟,练武,尹淑英,等.山楂原花青素调节环氧合酶-2表达诱导Eca109食管癌细胞凋亡[J].食品科学,2016,37(1):176-181.
[10] Zhang Y,Li D,Zhao X,et al.Decoy receptor 3 suppresses FasL-induced apoptosis via ERK1/2 activation in pancreatic cancer cells[J].Biochemical and Biophysical Research Communications,2015,463(4):1144-1151.
[11] Amin H,Wani N A,Farooq S,et al.Inhibition of Invasion in Pancreatic Cancer Cells by Conjugate of EPA with β(3,3)-Pip-OH via PI3K/Akt/NF-kB Pathway[J].ACS Medicinal Chemistry Letters,2015,6(10):1071-1074.
[12] Valentinos K,Georgios C,Michalis D M,et al.Microcystin LR Shows cytotoxic activity against pancreatic cancer cells expressing the membrane OATP1B1 and OATP1B3 transporters[J].Anticancer Research,2015, 35(11):5857-5865.
[13] Chang H H,Young S H,Sinnettsmith J,et al.Prostaglandin E2 activates the mTORC1 pathway through an EP4/cAMP/PKA and EP1/Ca2+-mediated mechanism in the human pancreatic carcinoma cell line PANC-1[J].American Journal of Physiology Cell Physiology,2015,309(10):639-649.
[14] Beaulieu J F. TuningWNT-β-catenin signaling via BCL9 proteins for targeting colorectal cancer cells[J].Ebiomedicine,2015,2(12):1846-1847.
[15] Anna G M,Malgorzata C.Modulation of WNT/β-catenin pathway in melanoma by biologically active components derived from plants[J].Fitoterapia,2016,109:283-292.
[16] Kumar S,Kumar D,Raina K,et al.Functional modification of adipocytes by grape seed extract impairs their pro-tumorigenic signaling on colon cancer stem cells and the daughter cancer cells[J].Oncotarget,2014,5(20):10151-10169.
[17] Prasad R,Katiyar S K.Grape seed proanthocyanidins inhibit migration potential of pancreatic cancer cells by promoting mesenchymal-to-epithelial transition and targeting NF-κB[J].Cancer Letters,2013,334(1):118-126.
[18] Zhang X X,Zhang L L,Yang H L,et al.Mechanism of Wnt/β-catenin signaling pathway in enhanced malignant phenotype of non-small cell lung cancer induced by anti-angiogenesis therapy[J].Asian Pacific Journal of Tropical Medicine,2016, 9(1):58-62. |
|
|
|
