GALNT12 对急性髓系白血病细胞生长影响的研究

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Abstract Objective To explore the effect of GALNT12 expression on the growth of acute myeloid leukemia cells.Methods The relevant data in the acute myeloid leukemia (AML) patients' database were analyzed to clearly explain the relationship between the expression level of GALNT12 mRNA and AML patients' survival.GALNT12 shRNA lentiviral vector was constructed and lentivirus containing GALNT12shRNA was packaged as a tool to construct GALNT12 silenced leukemia THP-1 cell line. The THP-1 cell line infected by the Scramble lentivirus was used as a control group to observe the difference in the growth of THP-1 cells between the silenced GALNT12 and the control group. GFP-positive cells were collected by flow cytometry and used to observe the effect of GALNT12 on the growth of AML cells.Results The expression of GALNT12 was negatively correlated with the survival cycle of AML patients and GALNT12 was highly expressed in acute myeloid leukemia THP-1 cell line. This study successfully constructed GALNT12 silenced leukemia THP-1 cell line.When GALNT12 shRNA1 interferenced THP-1 cells compared with shScramble,the growth rate was significantly slower.Conclusion The expression of GALNT12 can promote the growth of acute myeloid leukemia cells and promote the development of acute myeloid leukemia. It also adds new content to the effective molecular target for the treatment of acute myeloid leukemia.

Key words： GALNT12 acute myeloid leukemia cell growth shRNA

Received: 10 March 2017

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	ZHANG Qiang

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ZHANG Qiang. Effect identification of GALNT12 on the growth of acute myeloid leukemia cells[J]. 滨州医学院学报, 2017, 40(2): 117-119.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/ OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2017/V40/I2/117

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