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| Effect of bioactive glass 45S5 on odontogenic differentiation of apical papilla cells in vitro |
| CUI Caiyun1, DONG Yanmei2, QIU Wandi1, LIU Yusan1, WANG Wenhong3, LI Yanjun1,* |
1 Department of Oral Medicine, Binzhou Medical University Hospital, Binzhou 256603, Shandong, P. R. China;
2 Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology &National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratoryof Digital Stomatology, Beijing 10081, P. R. China;
3 Department of Pediatric Stomatology, Yantai Laishan Hospital of Stomatology, Yantai 264003, Shandong, P. R. China |
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Abstract Objective To determine the effect of bioactive glass 45S5 on odontoblast differentiation of human apical dental papilla cells in vitro.Methods The human apical papilla cells were co-cultured in the extracted culture medium with 0.1mg/mL 45S5. The inductively coupled plasma analysis was used to test the concentrations of silicon, calcium and phosphorus in the 45S5 extract. Cell proliferation was detected by thiazolyl blue colorimetry. Realtime-PCR was used to detect the expression of DSPP and DMP-1 genes related to odontogenic differentiation of apical papilla cells. With alizarin red mineralized nodule staining, acetylpyridine chloride calcium nodule was used to semi-quantitatively analyze the mineralized nodule formation of apical papilla cells.Results Compared with that in the control group, the concentration of silicon ion in 45S5 extraction medium increased significantly in the control group (P<0.05). The proliferation of apical dental papilla cells induced by 45S5 extract on the 3rd, 5th and 7th day was significantly higher than that in the control group (P<0.05). The expression of DSPP and DMP-1 in apical papilla cells induced by 45S5 extract for 7 days was higher than that in control group. Calcium acetylpyridine chloride nodules were semi-quantitatively analyzed for the formation of mineralized nodule of apical papilla cells in each group. OD values of silicon and OM groups were higher than those in the control group (P<0.05), the OD value of silicon group was higher than that of OM group (P<0.05).Conclusion Bioactive glass 45S5 in concentration of 0.1 mg/mL promotes the proliferation of apical papilla cells, the high expression of genes related to odontogenic differentiation and the formation of mineralized nodules in vitro. 45S5 promotes dentin differentiation of apical papilla cells in vitro.
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Received: 02 May 2021
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