基于16S rDNA高通量测序分析2型糖尿病腹泻患者肠道菌群的变化

doi:10.19739/j.cnki.issn1001-9510.2021.01.006

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Abstract Objective To study the changes of intestinal microflora in patients with type 2 diabetic diarrhea by 16S rDNA high-throughput sequencing technology.Methods Fecal samples of 18 patients with type 2 diabetic diarrhea and 23 healthy participantswere collected to test the intestinal flora genome. The second generation high-throughput gene sequencing technology was used to amplify and sequence the v3-v4 hypervariable region of 16S rDNA, and the bioinformatics analysis was carried out to compare the differences of intestinal flora structure and species between the two groups. Results There was no difference in age, gender and body mass index between the two groups. Alpha diversity analysis showed that the abundance and diversity of intestinal flora in patients with type 2 diabetic diarrhea were significantly lower than those in healthy controls, P<0.05. At the phylum level, the proportion of Bacteroides in the diarrhea patients with type 2 diabetes was significantly lower than that in the healthy group, P<0.05, while Firmicutes and verruca were significantly higher than those in the healthy group, with average P<0.05. In addition, the ratio of Firmicutes/Bacteroides in the patient group was significantly higher than those in the healthy group, P<0.05. At the genus level, fecobacteria in diarrhea patients with type 2 diabetes was significantly lower than that in healthy control group, P<0.05.Conclusion 16S rDNA high-throughput sequencing technology is helpful to analyze the diversity of intestinal flora in patients with type 2 diabetic diarrhea. This technology may provide novel ideas and theoretical basis for the study of the intestinal flora in patients with type 2 diabetic diarrhea.

Key words： 16S rDNA high-throughput sequencing type 2 diabetes mellitus diarrhea intestinal flora

Received: 13 August 2020

PACS:

R587.1

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	HAN Cuiyan
	SHI Ning

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HAN Cuiyan,SHI Ning. Changes of intestinal microflora in patients with type II diabetic diarrhea based on 16S rDNA high-throughput sequencing[J]. 滨州医学院学报, 2021, 44(1): 29-33.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.01.006 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I1/29

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