VEGF-B对胰岛β细胞胰岛素分泌和Ca<sup>2+</sup>、cAMP、ATP含量的影响

doi:10.19739/j.cnki.issn1001-9510.2021.01.004

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Abstract Objective To investigate the effects of VEGF-B on insulin secretion and intracellular Ca²⁺, cAMP and ATP content in pancreatic islet MIN6 cells and explore their possible mechanisms.Methods MIN6 cells were treated with 50ng/ml VEGF-B protein under the conditions of 5.5mM and 25mM glucose. ELISA kit was used to detect insulin secretion, intracellular Ca²⁺, cAMP and ATP content in MIN6 cells at 2 hours and 24 hours. After knocking down VEGF-B in MIN6 cells with siRNA for 48 hours, the insulin secretion, intracellular Ca²⁺, cAMP and ATP content of MIN6 cells were measured at 2 hours and 24 hours under the conditions of 5.5mM and 25mM glucose. Results After the intervention of VEGF-B in MIN6 cells, insulin secretion reduced, and the content ofCa²⁺, cAMPand ATP in the cells all decreased. After knocking down VEGF-B with siRNA, the secretion of insulin increased, and the content of Ca²⁺, cAMP and ATP in the cells were consistent with the change trend of insulin.Conclusion VEGF-B may affect the insulin secretion of pancreatic β-cells by affecting the content of Ca²⁺, cAMP and ATP.

Key words： VEGF-B MIN6 cell insulin secretion

Received: 25 November 2020

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R363

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	Articles by authors
	JIA Jingdan
	LUO Xu
	YU Hanpu
	ZHENG Lanhui
	LIU Wanchen
	WANG Zixu
	LI Ya'na

Cite this article:

JIA Jingdan,LUO Xu,YU Hanpu, et al. Effects of VEGF-B on insulin secretion and Ca²⁺, cAMP, ATP content of pancreatic β cells[J]. 滨州医学院学报, 2021, 44(1): 18-23.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.01.004 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I1/18

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