姜黄素促进炎症微环境下大鼠骨髓间充质干细胞骨向分化

doi:10.19739/j.cnki.issn1001-9510.2021.01.003

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Abstract Objective To study the effect of curcumin on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) under inflammatory microenvironment.Methods BMSCs were isolated and cultured, 10ng/ml TNF-α was used to induce cell inflammation, cells were treated with different concentrations of curcumin, and cell proliferation was detected by CCK-8 to select the appropriate concentration of curcumin for the experiment; the cells were divided into normal group, inflammation group and curcumin+inflammatory group; osteogenic differentiation was dectected with alkaline phosphatase (ALP) and alizarin red staining; the expression of bone sialoprotein (BSP) and runt-related transcription factor 2 (Runx2) was detected by real-time fluorescent quantitative PCR.Result Bone marrow mesenchymal stem cells were successfully isolated and cultured; the low concentration of curcumin had no cytotoxicity, and 1μmol /L was the optimum concentration. Inflammation decreased the expression of BSP and runx2, ALP staining and mineralized nodules were reduced; however, curcumin can reverse this trend and increase the expression of BSP and runx2 in the inflammatory state (P<0.05), and the ALP staining was deeper and the mineralized nodules increased compared with the inflammatory group.Conclusion Curcumin can promote osteogenic differentiation of bone marrow mesenchymal stem cells under inflammatory microenvironment.

Key words： curcumin inflammation BMSCs osteogenic differentiation

Received: 01 December 2020

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R965

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	Articles by authors
	DONG Chunyan
	LU Yongchao
	LIU Haiying
	XU Ziqi
	ZHANG Shaojun

Cite this article:

DONG Chunyan,LU Yongchao,LIU Haiying, et al. Curcumin promotes bone differentiation of rat bone marrow mesenchymal stem cells in inflammatory microenvironment[J]. 滨州医学院学报, 2021, 44(1): 14-17.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.01.003 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I1/14

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