黄杞苷对H<sub>2</sub>O<sub>2</sub>诱导SH-SY5Y细胞氧化应激损伤的保护作用

doi:10.19739/j.cnki.issn1001-9510.2021.01.001

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Abstract Objective To investigate the protective effect and molecular mechanism of engeletin on SH-SY5Y cells injured by H₂O₂.Methods SH-SY5Y cells were pretreated with engeletin (5, 10, 20, 40, 80 and 160 μmol·L^-1) for 12 h. MTT assay was used to detect the effect of engeletin on cell viability after H₂O₂ induced injury. The effects of engeletin on the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) after H₂O₂ induced injury were determined by NBT method and NADPH method; the levels of reactive oxygen species (ROS) in SH-SY5Y cells were determined by flow cytometry. SH-SY5Y cells were pretreated with engeletin (20 μmol·L^-1) for 12 h and incubated with H₂O₂ for 6 h, 12 h and 24 h, or pretreated with engeletin (5, 10 and 20 μmol·L^-1) for 12 h and incubated with H₂O₂ for 12 h. The expressions of Nrf2 and HO-1 were detected by Western blot. Results Engeletin could protect SH-SY5Y cells against H₂O₂ induced injury. Compared with the model group, engeletin could increase the cell viability, significantly increase the levels of SOD and GSH, and reduce the production of ROS (P<0.05). Engeletin promoted the transfer of Nrf2 protein from cytoplasm to nucleus, and reached the peak when SH-SY5Y cells were pretreated with engeletin (20 μmol·L^-1) for 12 h. In the model group of H₂O₂ induced injury, engeletin promoted the nuclear transfer of Nrf2 in a concentration dependent manner (P<0.05), and promoted the expression of HO-1, a downstream protein of Nrf2 (P<0.05).Conclusion Engeletin protected SH-SY5Y cells from H₂O₂ induced injury, and its mechanism was related to antioxidation and promoting the expression of Nrf2 and HO-1.

Key words： engeletin SH-SY5Y oxidative stress injury Nrf2, HO-1

Received: 04 December 2020

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R965

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	LIU Hui
	YAN Dong
	XU Yangyang
	LI Shucui
	ZHANG Shuping

Cite this article:

LIU Hui,YAN Dong,XU Yangyang, et al. Protective effect of engeletin on H₂O₂ induced oxidative stress injury in SH-SY5Y cells[J]. 滨州医学院学报, 2021, 44(1): 1-6.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2021.01.001 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2021/V44/I1/1

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