透明质酸与甲硝唑或米诺环素联合用药在实验性牙周炎中的作用机制

doi:10.19739/j.cnki.issn1001-9510.2020.05.009

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Abstract Objective To study the anti-inflammatory effect of hyaluronic acid combined with metronidazole or periocline on chronic periodontitis.Methods The periodontitis model of rats was established. According to the negative control group, the positive control group, the metronidazole group, the hyaluronic acid group, the metronidazole combined with hyaluronic acid group, the periocline group, and the periocline combined with hyaluronic acid group, the rats were divided into 7 groups, with 10 rats in each group. The expression levels of TNF-α and IL-1β protein in gingival crevicular fluid were measured by ELISA 2 h, 6 h, 24 h and 5 d after the treatment, then MMP8 and TIMP1 expressed in gingival epithelium were measured 24 h and 5 d after the treatment.Results Two hours after the treatment, the expression of TNF-α and IL-1 β in all experiment groups was significantly inhibited, P<0.01, especially in hyaluronic acid group, metronidazole combined with hyaluronic acid group, and periocline combined with hyaluronic acid group, P<0.01; 24 hours after the treatment, the inhibition of IL-1 β in metronidazole combined with hyaluronic acid group was significant; 5 days after the treatment, periocline combined with hyaluronic acid group still had a slow inhibition of TNF-α. Twenty-four hours after the treatment, MMP8 expression in all experiment groups showed a downward trend, P<0.01, while the expression of TIMP1 significantly increased, P<0.01, especially in metronidazole combined with hyaluronic acid group, periocline group and periocline combined with hyaluronic acid group; 5 days after the treatment, the expression of TIMP1 in Metronidazole combined with hyaluronic acid group showed an upward trend, P<0.01.Conclusion Local application of metronidazole, hyaluronic acid and Periocline has obvious effect on the early stage of inflammation, but the long-term maintenance effect is not good; the combination of metronidazole and hyaluronic acid, periocline and hyaluronic acid is better than single application. In contrast, the combination of metronidazole and hyaluronic acid has more obvious advantages in five-day continuous anti-inflammatory effect.

Key words： chronic periodontitis hyaluronic acid metronidazole periocline

Received: 28 April 2020

PACS:

R781.4

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	Articles by authors
	GUO Wei
	WANG Peng
	ZHENG Mingxue
	WANG Kun
	GAO Shuting
	YANG Yan

Cite this article:

GUO Wei,WANG Peng,ZHENG Mingxue, et al. Experimental study of hyaluronic acid combined with metronidazole
or periocline in the treatment of chronic periodontitis[J]. 滨州医学院学报, 2020, 43(5): 353-358.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.05.009 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I5/353

[1] MIANI P K,DO NASCIMENTO C,SATO S,et al.In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronicperiodontitis: preliminary results[J].Eur J Clin Microbiol Infect Dis,2012,31(7): 1611-1618.
[2] REISE M,WYRWA R,M LLER U,et al.Release of metronidazole from electrospun poly(L-lactide-co-D/L-lactide) fibers for local periodontitis treatment[J].Dent Mater,2012,28(2): 179 -188.?
[3] 李洪军.牙周缓释剂治疗侵袭性牙周炎的临床疗效观察[J].全科口腔医学杂志,2017,17(4):16-17.
[4] VANHEUSDEN A,NUSGENS B,GOFFINET G,et al.In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL[J].J Periodontal Res,1998,33(6): 377-385.
[5] 刘佩娟.牙周局部缓释药物治疗牙周炎的应用研究进展 [J].临床医药文献杂志,2017,69(4):13658.
[6] 刘鹬.派丽奥与牙康治疗牙周炎的效果观察[J].全科口腔医学电子杂志,2019,6(26):62-64.
[7] 李允允,法永红.透明质酸在口腔领域中的应用进展[J].国际口腔医学杂志,2013,40(3):344-346.
[8] EICK S,RENATUS A,HEINICKE M,et al.Hyaluronic Acid as an adjunct after scaling and root planing: a prospective randomized clinical trial [J].J Periodontol,2013,84(7): 941-949.
[9] FISCHER R G,KLING B.Clinical and histological evaluation of ligature-induced periodontitis in the domestic ferret [J].J Clin Perionont,1994,21(4): 230
[10] 金岩.实现牙周组织形态与功能重建是牙再生的关键科学问题[J].口腔医学,2015,35(8):609-613
[11] 刘学玉,潘克清,张丽.羧甲基壳聚糖锌多肽复合材料局部应用对巴马小型猪龈沟液中IL-1、TNF-α和PGE-2含量的影响[J].上海口腔医学,2016,25(2):172-176.
[12] 路瑞芳,冯向辉,徐莉,等.牙周基础治疗对侵袭性牙周炎患者唾液和龈沟液中牙周致病菌的作用[J].中华口腔医学杂志,2012,47(11):11-15.
[13] GEMMELL E 1,MARSHALL R I,SEYMOUR G J.Cytokines and prostaglandins in immune homeostasis and tissue destruction in periodontal disease[J].Periodontol,1997,14: 112-143.
[14] 田江雪,莫龙义,贾小玥,等.转化生长因子β在牙周炎发生发展中的作用及其机制[J].国际口腔医学杂志,2018,45(5):553-559.
[15] 杨丕山,宋爱梅.TNF-α/NF-κB 信号通路对牙周炎发展和牙周再生的影响及其干预[J].口腔医学,2019,39(1):1 -5.
[16] REPEKE C E,CARDOSO C R,CLAUDINO M, et al.Non-inflammatory destructive periodontal disease: a clinical,microbiological,immunological and genetic investigation[J].J Appl Oral Sci,2012,20(1): 113-121.
[17] 邓咏梅,王辉,何松霖,等.盐酸米诺环素纳米脂质体治疗大鼠实验性牙周炎的疗效观察[J].实用口腔医学杂志,2018,34(6):753-757.
[18] 李湘明.甲硝唑联合盐酸米诺环素治疗牙周病的临床观察[J].中国现代医生,2010,48(21):56-57.
[19] DAHIYA P,KAMAL R.Hyaluronic Acid: a boon in periodontal therapy[J].N Am J Med Sci,2013,5(5): 309-315.
[20] 何春丽.Gengigel凝胶联合浓替硝唑含漱液在菌斑性牙龈炎患者中的治疗作用探讨[J].黑龙江中医药,2019,(2):306-307.
[21] YE S.Putative targeting of matrix metalloproteinase-8 in atherosclerosis[J].Pharmacol Ther,2015,147: 111-122.
[22] 高颖,郝晨笛.慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系[J].上海口腔医学,2018,27(3):309-312.