翻白草总黄酮的制备及抗炎活性研究

doi:10.19739/j.cnki.issn1001-9510.2020.02.013

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Abstract Objective To study on the preparation process and anti-inflammatory activity of total flavonoids of PotentilladiscolorBge. Methods The plant was extracted with 70% ethanol, and purified with polyamide resin, and rutin was used as a control to investigate the distribution and purity of total flavones.Mononuclear macrophage RAW264.7 was induced with lipopolysaccharide (LPS), and after the treatment with different concentrations (25, 50, 100 μg·mL^-1) of total flavonoids of PotentilladiscolorBge., quantitative PCR was used to detect the mRNA expression levels of inflammatory factors iNOS, IL-1β, and IL-6. Results The purity of total flavonoids of PotentilladiscolorBge. obtained by polyamide chromatography was 87.4%, and the yield was 82.6%. The total flavones of PotentilladiscolorBge. could significantly down-regulate the mRNA expression of inflammatory factors iNOS, IL-1β and IL-6 in RAW 264.7 cells induced by LPS. Conclusion The total flavonoids of PotentilladiscolorBge. can be prepared by polyamide resin chromatography, and its anti-inflammatory activity is related to the down-regulation of mRNA expression of iNOS, IL-1β, and IL-6.

Key words： PotentilladiscolorBge. total flavonoid preparation process anti-inflammatory

Received: 28 June 2019

PACS:

R285.5

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	Articles by authors
	YU Zeqiu
	ZHAO Ying
	SHI Wenxing
	QU Guiwu

Cite this article:

YU Zeqiu,ZHAO Ying,SHI Wenxing, et al. Study on preparation process and anti-inflammatory activity of total flavonoids of PotentilladiscolorBge.[J]. 滨州医学院学报, 2020, 43(2): 132-135.

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http://manu58.magtech.com.cn/Jwk_bzyxy/EN/10.19739/j.cnki.issn1001-9510.2020.02.013 OR http://manu58.magtech.com.cn/Jwk_bzyxy/EN/Y2020/V43/I2/132

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