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| Effect of ursolic acid derivatives on bone remodeling in diabetic osteoporosis |
| ZHAO Yuqin1, ZHANG Chengjie1, XIANG Shufang2, |
1 Department of Emergency, Binzhou People's Hospital, Binzhou 256610,P.R.China;
2 Department of Obstetrics, Binzhou People's Hospital |
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Abstract Objective The aim of this study was to investigate the effects of ursolic acid derivatives (UAD) on bone remodeling in diabetic osteoporosis. Methods The mice were randomly divided into three groups: control group, STZ group and US group.The tibia was taken for gene and protein expression determination and histomorphologicalanalysis. Bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and bone mineral density/total volume (BMD/TV) were measured.Detection of osteoprotegerin/nuclear factor-kappa B receptor activator (OPG/RANKL) ratio and expression of key regulators of bone metabolism. Results Compared with the control group, OPG/RANKL ratio and OPG in STZ group decreased significantly and RANKL increased significantly.The OPG/RANKL ratio increased significantly in US group.The expression of MMP9 and CAI in STZ group was significantly higher than that in control group, while the expression of Runx2 and TGF-beta in STZ group was significantly lower than that in control group.US group reversed the expression of MMP9, CAII, RUNX2 and TGF-beta in diabetic mice at the same time. Conclusion UAD reverses STZ-induced trabecular bone injury by promoting osteoblast differentiation, new bone formation and inhibiting osteoclast bone resorption.
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Received: 13 May 2019
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