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| Determination of total flavonoids of Chrysanthemum indicum extract |
| SONG Dehai1, XU Qianqian2,3, SUN Yingdong1, LI Baoyuan1, GUO Shijin2,3, ZHANG Jinjie1 |
1 Department of Pharmacy,Binzhou Medical University Hospital,Binzhou 256603,P.R.China;
2 Binzhou Animal Science and Veterinary Medicine Academy;
3 Lvdu Ante Veterinary Drug Industry |
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Abstract Objective To determine the total flavonoids content of Chrysanthemum indicum extract by polyamide column. Methods The sample of Chrysanthemum indicum was heated to reflux by 60% ethanol to get the crude extract.The extract was purified by polyamide chromatography column with different concentrations of ethanol,and the content of the total flavonoids in different eluent layers was determined by UV-Vis spectrophotometry.Results The yield of 50% ethanol eluent in Chrysanthemum indicum extract was 0.9753% and the dry ointment rate was the highest. The content of flavonoids in 70% ethanol eluent was 46.51% and the content was the highest.Conclusion The method using polyamide chromatography column is accurate, sensitive, simple and reproducible,and it provides a scientific basis for the enrichment and purification of flavonoids of Chrysanthemum indicum.
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Received: 08 June 2018
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