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| The effects of miRNA-132 on proliferation and apoptosis of leukemia cell line K562 |
| FAN Yunxiu, LI Jianchang, SUN Weimei, CHEN Bo |
| Department of Pediatrics,Binzhou Medical University Hospital,Binzhou 256603,P.R.China |
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Abstract Objective To investigate the effect of miRNA-132 on proliferation and apoptosis of leukemia cell line K562, and explore its possible mechanism.Methods K562 cells were transfected with LipofectamineTM 2000.The expression of miRNA-132 was detected by real-time quantitative PCR.The effect of transfection on proliferation and apoptosis of K562 cells was detected by CCK-8 and flow cytometry.The protein expression levels of SRIT1 and P53 were detected by Western Blot.Results The expression of miRNA-132 in normal cells was significantly lower than that in K562 cells.Compared with the blank group and the negative control group,the expression of miRNA-132 was significantly increased in the transfected group.K562 cells’ proliferated and the apoptosis rate decreased significantly after transfection of miRNA-132 mimics.Compared with the blank control group and miRNA-132-NC group,after the high expression of miRNA-132,the expression of SIRT1 protein in the transfected group was significantly decreased,and the expression of P53 protein was significantly increased.Conclusion Highly expression of miRNA-132 significantly inhibits the proliferation of leukemia K562 cells and induces the apoptosis,which may be related to the enhancement of SIRT1 and P53 activity.
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Received: 01 October 2018
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